Delayed but sustained induction of mitogen-activated protein kinase activity is associated with b-adrenergic receptor-mediated morphological differentiation of astrocytes
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Delayed but sustained induction of mitogen-activated protein
kinase activity is associated with b-adrenergic receptor-mediated
morphological differentiation of astrocytes
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| Creator |
Gharami, Kusumika
Das, Sumantra |
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| Subject |
Chemistry
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| Description |
Astroglial b-adrenergic receptors (b-ARs) are functionally
linked to regulate cellular morphology. In primary cultures, the
b-AR agonist isoproterenol (ISP) can transform flat polygonal
astrocytes into process-bearing, mature stellate cells by 48 h,
an effect that can be blocked by the b-AR antagonist, propranolol.
ISP induced immediate activation of protein kinase A
(PKA) which persisted up to 2 h, with no visible change in cell
morphology. However, activation of PKA was sufficient to
drive the process of transformation to completion, suggesting
the involvement of downstream regulators of PKA. In addition
to PKA inhibitors, the mitogen-activated protein kinase
(MAPK) kinase inhibitor PD098059 also blocked ISP-induced
morphological transformation. ISP treatment resulted in a
biphasic response of cellular phosphorylated MAPK (phosphorylated
extracellular signal-regulated kinase; p-ERK) level:
an initial decline in p-ERK level followed by a sustained
induction at 12–24 h, both of which were blocked by PKA
inhibitor. The induction in pERK level coincided with initiation
of morphological differentiation of the astrocytes and nuclear
translocation of p-ERK. A long-lasting activation of p-ERK
activity by ISP, at a later stage, appears to be critical for the
transformation of astrocytes.
Keywords: astrocyte, b-adrenergic receptors, mitogen-activated
protein kinase, morphology, protein kinase A, protein
kinase C.
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| Date |
2004
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/446/1/JOURNAL_OF_NEUROCHEMISTRY%2C_88(_1)%2C_12%2D22[93].pdf
Gharami, Kusumika and Das, Sumantra (2004) Delayed but sustained induction of mitogen-activated protein kinase activity is associated with b-adrenergic receptor-mediated morphological differentiation of astrocytes. Journal of Neurochemistry, 88 (1). pp. 12-22. |
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| Relation |
http://dx.doi.org/10.1046/j.1471-4159.2003.02148.x
http://www.eprints.iicb.res.in/446/ |
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