Mahanine InhibitsGrowth and InducesApoptosis in ProstateCancerCellsThroughtheDeactivation of Akt andActivation ofCaspases
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Mahanine InhibitsGrowth and InducesApoptosis in
ProstateCancerCellsThroughtheDeactivation of
Akt andActivation ofCaspases
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| Creator |
Sinha, Swati
Pal, Bikas C Jagadeesh, Shankar Banerjee, Partha P Bandyopadhaya, Arun Bhattacharya, Samir |
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| Subject |
Cell Biology & Physiology
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| Description |
The present study was undertaken to evaluate anti-proliferative and -
apoptotic activities of mahanine, a plant derived carbazole alkaloid, in prostate cancer cells and
to determine its molecular mechanism by which it induces apoptotic cell death.
The growth inhibitory and apoptotic inductive effect of mahanine on prostate
cancer cells were examined by measuring cell proliferation and BrdU labeling, caspase activity,
DNA fragmentation, and Western blot analyses.
Mahanine inhibited growth of PC3 and LNCaP prostate cancer cells in a dose and
time-dependent manner. Mechanistically, mahanine inhibited cell-survival pathway by
dephosphorylation of PIP3 dependent kinase 1 (PDK1) thereby deactivation of Akt and
downregulation of Bcl-xL. In addition, mahanine activated caspase pathway (caspases 9 and 3)
and eventually cleavage of DNA repair enzyme, PARP resulting DNA fragmentation and
apoptosis.
Mahanine inhibits growth and induces apoptosis in both androgenresponsive,
LNCaP and androgen-independent, PC3 cells by targeting cell survival pathway.
Prostate 66: 1257–1265, 2006. #2006 Wiley-Liss, Inc.
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| Date |
2006
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/483/1/PROSTATE%2C__66(_12)%2C_1257%2D1265_[41].pdf
Sinha, Swati and Pal, Bikas C and Jagadeesh, Shankar and Banerjee, Partha P and Bandyopadhaya, Arun and Bhattacharya, Samir (2006) Mahanine InhibitsGrowth and InducesApoptosis in ProstateCancerCellsThroughtheDeactivation of Akt andActivation ofCaspases. The Prostate, 66 (12). pp. 1257-1265. |
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| Relation |
http://dx.doi.org/
http://www.eprints.iicb.res.in/483/ |
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