Small Cationic Protein From a Marine Turtle Has �- Defensin-Like Fold and Antibacterial and Antiviral Activity
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Small Cationic Protein From a Marine Turtle Has �-
Defensin-Like Fold and Antibacterial and Antiviral Activity
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| Creator |
Chattopadhyay, Suranjana
Sinha, Nirmal Kumar Banerjee, Shuvojit Roy, Debjani Chattopadhyay, Dhrubajyoti Roy, Siddhartha |
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| Subject |
Structural Biology & Bioinformatics
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| Description |
Egg white of marine turtle Caretta
caretta contains a small cationic protein but lacks
lysozyme. The protein was sequenced by a combination
of sequential Edman degradation, carboxypeptidase
digestion, nuclear magnetic resonance (NMR)
and electrospray ionization tandem mass spectrometry.
The protein contains 36 amino acid residues of
which six are half-cysteines. The three-dimensional
structure of the protein was deduced from twodimensional
NMR experiments and was observed to
be similar to vertebrate �-defensins. However, disulfide
connectivity is C1–C6/C2–C5/C3–C4; different
from that of the vertebrate �-defensins. The protein
showed strong antibacterial activity against Escherichia
coli and Salmonella typhimurium. The protein
also showed significant antiviral activity against
an enveloped rhabdovirus, Chandipura virus, which
is an emerging human pathogen. This virus is also
closely related to the vesicular stomatitis virus,
whose growth was also inhibited. This small cationic
protein is part of the innate immunity of this
organism and replaces lysozyme in the egg. It has
the potential to be developed as an antibacterial
and antiviral agent. Proteins 2006;64:524–531.
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| Date |
2006
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/485/1/PROTEINS%2DSTRUCTURE_FUNCTION_AND_BIOINFORMATICS%2C_64(_2)%2C524%2D531%2C[52].pdf
Chattopadhyay, Suranjana and Sinha, Nirmal Kumar and Banerjee, Shuvojit and Roy, Debjani and Chattopadhyay, Dhrubajyoti and Roy, Siddhartha (2006) Small Cationic Protein From a Marine Turtle Has �- Defensin-Like Fold and Antibacterial and Antiviral Activity. "Proteins: Structure, Function, and Bioinformatics", 64 (2). pp. 524-531. |
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| Relation |
http://dx.doi.org/10.1002/prot.20963
http://www.eprints.iicb.res.in/485/ |
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