A naturally occurring point mutation in the 13-mer R repeat affects the oriCfunction of the large chromosome of Vibrio choleraeO1 classical biotype
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
A naturally occurring point mutation in the 13-mer R repeat affects
the oriCfunction of the large chromosome of Vibrio choleraeO1
classical biotype
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| Creator |
Saha, Arjun
Haralalka, Shruti Bhadra, Rupak K |
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| Subject |
Infectious Diseases and Immunology
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| Description |
The genome of Vibrio cholerae consists of
two circular chromosomes of di.erent sizes. Here, a
comparative analysis of the replication origins of the
large chromosomes (oriCIVC) of classical and El Tor
biotypes of the pathogen is reported. Extensive nucleotide
sequence analyses revealed that the oriCIVC region
has six DnaA boxes instead of the .ve found in Escherichia
coli oriC. The additional DnaA box, designated
RV, was unique in V. cholerae as well as in other members
of the family Vibrionaceae. However, RV was not
found to be essential for the autonomous replication
function of the 307-bp oriCIVC minimal region. In contrast
to El Tor and the recently evolved V. cholerae O139
strains, the oriCIVC region of the classical biotype
showed only a single base transition (T . G) in a highly
conserved AT-rich 13-mer R repeat region. From the
minichromosome copy number and its transformational
e.ciency analyses, it appears that the single base substitution
in the oriCIVC of the classical biotype has a
signi.cant e.ect on its replication initiation.
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| Date |
2004
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/498/1/ARCHIVES_OF_MICROBIOLOGY%2C_182(_5)%2C__421%2D427_[10].pdf
Saha, Arjun and Haralalka, Shruti and Bhadra, Rupak K (2004) A naturally occurring point mutation in the 13-mer R repeat affects the oriCfunction of the large chromosome of Vibrio choleraeO1 classical biotype. Arch Microbiol , 182 (5). pp. 421-427. |
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| Relation |
http://dx.doi.org/10.1007/s00203-004-0708-y
http://www.eprints.iicb.res.in/498/ |
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