Differential Expression of 9-o-aAcetylated Sialoglycoconjugates on Leukemic blasts: a Potential tool for long-term Monitoring of Children with Acute Lymphoblastic Leukemia
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Differential Expression of 9-o-aAcetylated Sialoglycoconjugates on Leukemic blasts: a Potential tool for long-term Monitoring of Children with Acute Lymphoblastic Leukemia
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| Creator |
Pal, Santanu
Ghosh, Shyamasree Bandyopadhyay, Suman Mandal, Chhabinath Bandyopadhyay, Santu Bhattacharyya, Dilip Kumar Mandal, Chitra |
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| Subject |
Drug Development/Diagnostics & Biotechnology
Infectious Diseases and Immunology |
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| Description |
Earlier studies have demonstrated overexpression of 9-Oacetylated
sialoglycoconjugates (9-O-AcSGs) on lymphoblasts,
concomitant with high titers of anti-9-O-AcSG antibodies
in childhood acute lymphoblastic leukemia (ALL).
Our aim was to evaluate the correlation between expression
of different 9-O-AcSGs during chemotherapeutic treatment.
Accordingly, expression of 9-O-AcSGs on lymphoblasts of
ALL patients (n � 70) were longitudinally monitored for 6
years (1997–2002), using Achatinin-H, a 9-O-acetylated sialic
acid (9-O-AcSA) binding lectin with preferential affinity for
9-O-AcSGs with terminal 9-O-AcSA�236GalNAc. Western
blot analysis of patients (n � 30) showed that 3 ALL-specific
9-O-AcSGs (90, 120 and 135 kDa) were induced at presentation;
all these bands disappeared after treatment in patients
(n � 22) who had disease-free survival. The 90 kDa band
persisted in 8 patients who subsequently relapsed with reexpression
of the 120 kDa band. FACS analysis revealed that at
presentation (n � 70) 90.1 � 5.0% cells expressed 9-O-AcSGs,
which decreased progressively with chemotherapy, remained
<5% during clinical remission and reappeared in relapse
(80 � 10%, n � 18). Early clearance of 9-O-AcSG� cells,
during 4–8 weeks of treatment showed a good correlation
with low risk of relapse. Sensitivity of detection of 9-OAcSG
� cells was 0.1%. Numbers of both high- and low-affinity
binding sites were maximum at presentation, decreased with
treatment and increased again in clinical relapse. We propose
that close monitoring of 90 and 120 kDa 9-O-AcSGs
may serve as a reliable index for long-term management of
childhood ALL and merits therapeutic consideration.
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| Publisher |
John Wiley & Sons
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| Date |
2004
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/537/1/INTERNATIONAL_JOURNAL_OF_CANCER%2C_111(_2)%2C_270%2D277_[35].pdf
Pal, Santanu and Ghosh, Shyamasree and Bandyopadhyay, Suman and Mandal, Chhabinath and Bandyopadhyay, Santu and Bhattacharyya, Dilip Kumar and Mandal, Chitra (2004) Differential Expression of 9-o-aAcetylated Sialoglycoconjugates on Leukemic blasts: a Potential tool for long-term Monitoring of Children with Acute Lymphoblastic Leukemia. International Journal of Cancer, 111 (2). pp. 270-277. ISSN 0020-7136 |
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| Relation |
http://dx.doi.org/10.1002/ijc.20246
http://www.eprints.iicb.res.in/537/ |
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