“Ping-Pong” Interactions between Mitochondrial tRNA Import Receptors within a Multiprotein Complex
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
“Ping-Pong” Interactions between Mitochondrial tRNA Import
Receptors within a Multiprotein Complex
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| Creator |
Bhattacharyya, Suvendra Nath
Chatterjee, Saibal Goswami, Srikanta Tripathi, Gayatri Dey, Sailendra Nath Adhya, Samit |
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| Subject |
Molecular & Human Genetics
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| Description |
The mitochondrial genomes of a wide variety of species contain an insufficient number of functional tRNA
genes, and translation of mitochondrial mRNAs is sustained by import of nucleus-encoded tRNAs. In Leishmania,
transfer of tRNAs across the inner membrane can be regulated by positive and negative interactions
between them. To define the factors involved in such interactions, a large multisubunit complex (molecular
mass, �640 kDa) from the inner mitochondrial membrane of the kinetoplastid protozoon Leishmania, consisting
of �130-A° particles, was isolated. The complex, when incorporated into phospholipid vesicles, induced
specific, ATP- and proton motive force-dependent transfer of Leishmania tRNATyr as well as of oligoribonucleotides
containing the import signal YGGYAGAGC. Moreover, allosteric interactions between tRNATyr and
tRNAIle were observed in the RNA import complex-reconstituted system, indicating the presence of primary
and secondary tRNA binding sites within the complex. By a combination of antibody inhibition, photochemical
cross-linking, and immunoprecipitation, it was shown that binding of tRNAIle to a 21-kDa component of the
complex is dependent upon tRNATyr, while binding of tRNATyr to a 45-kDa component is inhibited by tRNAIle.
This “ping-pong” mechanism may be an effective means to maintain a balanced tRNA pool for mitochondrial
translation.
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| Date |
2003
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/555/1/MOLECULAR_AND_CELLULAR_BIOLOGY%2C_23(15)%2C_5217%2D5224[39].pdf
Bhattacharyya, Suvendra Nath and Chatterjee, Saibal and Goswami, Srikanta and Tripathi, Gayatri and Dey, Sailendra Nath and Adhya, Samit (2003) “Ping-Pong” Interactions between Mitochondrial tRNA Import Receptors within a Multiprotein Complex. MOLECULAR AND CELLULAR BIOLOGY, 23 (15). pp. 5217-5224. |
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| Relation |
http://dx.doi.org/10.1128/MCB.23.15.5217–5224.2003
http://www.eprints.iicb.res.in/555/ |
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