Interference of laccase in determination of cellobiose dehydrogenase activity of Pleurotus ostreatus (Florida) using dichlorophenol indophenol as the electron acceptor
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Interference of laccase in determination of cellobiose
dehydrogenase activity of Pleurotus ostreatus (Florida)
using dichlorophenol indophenol as the electron acceptor
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| Creator |
Saha, Tanima
Chakraborty, Tapas Kumar Saha, Rina Das, N Mukherjee, Mina |
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| Subject |
Drug Development/Diagnostics & Biotechnology
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| Description |
Pleurotus ostreatus (Florida), ITCC 3308 produces ~9.0 U/ml extracellular cellobiose dehydrogenase
(CDH) in cellulose medium after 7 days of growth. However, no activity could be detected if the assay
was done with cellobiose as the substrate and 2,6-dichlorophenol indophenol (DPIP) as the electron
acceptor in absence of any laccase inhibitor. Kinetic study showed that Vmax/Km value was very high
for rDPIP (reduced 2,6-dichlrophenol indophenol) oxidation by laccase. Oxygen consumption rate of
rDPIP oxidation by the enzyme was found to be highest among all the tested substrates. The present
study indicated that rDPIP was a good substrate for laccase. Therefore, caution is needed to measure
CDH activity by monitoring DPIP reduction in a system where laccase is likely to be present.
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| Publisher |
John Wiley & Sons
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| Date |
2005
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/577/1/JOURNAL_OF_BASIC_MICROBIOLOGY__45(_2)142%2D146;2005[109].pdf
Saha, Tanima and Chakraborty, Tapas Kumar and Saha, Rina and Das, N and Mukherjee, Mina (2005) Interference of laccase in determination of cellobiose dehydrogenase activity of Pleurotus ostreatus (Florida) using dichlorophenol indophenol as the electron acceptor. Journal of Basic Microbiology, 45 (2). pp. 142-146. ISSN 0233-111X |
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| Relation |
http://dx.doi.org/10.1002/jobm.200410472
http://www.eprints.iicb.res.in/577/ |
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