Accelerated Publication Role of superoxide dismutase in survival of Leishmania within the macrophage
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Accelerated Publication
Role of superoxide dismutase in survival of Leishmania within
the macrophage
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| Creator |
Ghosh, Sanjay
Goswami, Srikanta Adhya, Samit |
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| Subject |
Molecular & Human Genetics
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| Description |
Intracellular parasitic protozoans of the genus Leishmania depend
for their survival on the elaboration of enzymic and other
mechanisms for evading toxic free-radical damage in¯icted by
their phagocytic macrophage host. One such mechanism may
involve superoxide dismutase (SOD), which detoxi®es reactive
superoxide radicals produced by activated macrophages, but the
role of this enzyme in parasite survival has not yet been
demonstrated. We have cloned a SOD gene from L. tropica and
generated SOD-de®cient parasites by expressing the corresponding antisense RNA from an episomal vector. Such parasites have
enhanced sensitivity to menadione and hydrogen peroxide in
axenic culture, and a markedly reduced survival in mouse
macrophages. These results indicate that SOD is a major
determinant of intracellular survival of Leishmania.
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| Publisher |
Portland Press
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| Date |
2003
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/593/1/BIOCHEMICAL_JOURNAL%2C369%2C_447%2D452[82].pdf
Ghosh, Sanjay and Goswami, Srikanta and Adhya, Samit (2003) Accelerated Publication Role of superoxide dismutase in survival of Leishmania within the macrophage. Biochemical Journal, 369. pp. 447-452. ISSN 0264-6021 |
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| Relation |
http://dx.doi.org/10.1042/BJ20021684
http://www.eprints.iicb.res.in/593/ |
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