Lectin like properties and differential sugar binding characteristics of C-reactive proteins puri®ed from sera of normal and pollutant induced Labeo rohita
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Lectin like properties and differential sugar binding
characteristics of C-reactive proteins puri®ed from
sera of normal and pollutant induced Labeo rohita
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| Creator |
Mandal, Chitra
Sinha, Sukanya Mandal, Chhabinath |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Different forms of C-reactive proteins (CRPs) have been puri®ed to electrophoretic homogeneity from the sera of Labeo
rohita con®ned in freshwater (CRPN) and water polluted with nonlethal doses of cadmium (CRPCd) or mercury (CRPHg).
CRPN , CRPCd , and CRPHg show remarkable differences in their electrophoretic mobility but exhibit strong immunological
cross reactivity. All these CRPs exhibit variable agglutination properties with erythrocytes from diverse sources in
presence of Ca�2, which could be inhibited by a variety of sugars showing speci®city for galactose. Inhibition results
show that the potency of galactose as an inhibitor increases about 4 fold in the process of transformation of CRPN to
CRPCd and CRPHg . In case of CRPN , Gal b(1!1) Gal and oNO2 phenyl b-Gal show highest inhibitory potency while oNO2-
phenyl b-Gal is the most potent inhibitor for CRPCd and CRPHg but the potency of Gal b(1!1) Gal reduced drastically. 6-
phosphate D-Gal and stachyose are 20 times weaker inhibitors than D-Gal for induced CRP mediated agglutination, in
contrast, these sugars are only 6 times weaker for CRPN . Dissociation constants of the binding of CRPN with phosphoryl
choline (PC) and galactose are about 9mM and PC binding causes a change in the a and b conformations of these CRPs.
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| Publisher |
Kluwer
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| Date |
1999
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/669/1/GLYCOCONJUGATE_JOURNAL16(11)741%2D750;1999[11].pdf
Mandal, Chitra and Sinha, Sukanya and Mandal, Chhabinath (1999) Lectin like properties and differential sugar binding characteristics of C-reactive proteins puri®ed from sera of normal and pollutant induced Labeo rohita. Glycoconjugate Journal, 6 (11). pp. 741-750. |
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| Relation |
http://dx.doi.org/10.1023/A:1007167611778
http://www.eprints.iicb.res.in/669/ |
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