Integration of the DNA of a novel filamentous bacteriophage VSK from Vibrio cholerae 0139 into the host chromosomal DNA
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Integration of the DNA of a novel filamentous bacteriophage
VSK from Vibrio cholerae 0139 into the host chromosomal DNA
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| Creator |
Kar, Sudeshna
Ghosh, Ranajit K Ghosh, Amar N Ghosh, Amit |
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| Subject |
Chemistry
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| Description |
An unusual lilamentous bacteriophage, VSK, containing single-stranded, circular DNA as its genome was isolated from Vibuio cholerae 0139 strains PO7 and B04. Unlike other single-stranded DNA phages, VSK can integrate its genome into the chromosome of the host and enter into a lysogenic state. The double-stranded replicative form (RF) of the single-stranded phage DNA was isolated. A restriction map of the VSK RF DNA was constructed using HaeII, AvnII, ClaI and XbaI. By Southern blot analysis of the chromosomal DNA of the lysogen using labeled phage DNA as probe, the attachment site (attP) on the viral genome was also identified.
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| Date |
1996
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/707/1/FEMS_MICROBIOLOGY_LETTERS%2C145(_1)%2C__17%2D22_[6].pdf
Kar, Sudeshna and Ghosh, Ranajit K and Ghosh, Amar N and Ghosh, Amit (1996) Integration of the DNA of a novel filamentous bacteriophage VSK from Vibrio cholerae 0139 into the host chromosomal DNA. FEMS Microbiology Letters, 145 (1). pp. 17-22. |
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| Relation |
http://dx.doi.org/037X-1097/96
http://www.eprints.iicb.res.in/707/ |
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