In-vitro initiation of forward motility in testicular spermatozoa
IR@IICB: CSIR-Indian Institute of Chemical Biology, KolkataView Archive Info
In-vitro initiation of forward motility in testicular
Jaiswal, B S
Majumder, Gopal Chandra
Initiation of forward mothty in vitro was investigated in goat and ram spermatozoa obtained from the rete testis. No forward motility was generated in the immotile
testicular spermatozoa when they were incubated in a modified Ringer’s solution containing theophyhe (30 mM) and epididymal plasma (2 mg protein/ml). However,
these reagents induced non-progressive flagellar movement in approximately 25% of spermatozoa. Bicarbonate (25 mM) induced forward motility in approximately 16% of
the goadram testicular spermatozoa. Theophylline was essential for the bicarbonate- mediated activation of sperm motility, but epididymal plasma had no significant effect on this activation process. Theophylline activated progressive motility in testicular spermatozoa in a dose-dependent manner, the maximum effect occurring after
incubation for 10 min with 30 mM theophylline. The initiation profile of in-vitro motility of goadram spermatozoa from the caput epidldymis closely resembled that of testicular spermatozoa except that induction of motihty in the caput spermatozoa was dependent both on bicarbonate and epiddymal plasma. The data indcate that, unlike caput epidldymal spermatozoa, initiation of motility in testicular spermatozoa is not dependent on motility-promoting protein(s) in epidldymal plasma.
Jaiswal, B S and Majumder, Gopal Chandra (1996) In-vitro initiation of forward motility in testicular spermatozoa. International Journal of Andrology, 19 (2). pp. 97-102.