DNA extraction from crayfish exoskeleton
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Creator |
Li, Yanhe
Wang, Weimin Liu, Xiaolian Luo, Wei Zhang, Jie Gul, Yasmeen |
|
| Date |
2011-11-30T09:34:54Z
2011-11-30T09:34:54Z 2011-12 |
|
| Identifier |
0975-1009 (Online); 0019-5189 (Print)
http://hdl.handle.net/123456789/13094 |
|
| Description |
953-957
Crayfish exoskeleton (CE) samples are generally less invasive and easy to be collected. However, it is difficult to extract DNA from them. This study was intended to investigate CE as a DNA source and design an easy and efficient DNA extraction protocol for polymerase chain reactions. Specific primer pair (PPO-F, PPO-R) was used to amplify extracted DNA from CE, and compared to crayfish tail muscle DNA sample. Moreover, seven microsatellites markers were used to amplify the CE DNA samples set. Since the extracted DNA from CE is suitable for gene amplification, the results present usefulness of CE as an easy and convenient DNA source for PCR-based population genetic research. |
|
| Language |
en_US
|
|
| Publisher |
NISCAIR-CSIR, India
|
|
| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
|
|
| Source |
IJEB Vol.49(12) [December 2011]
|
|
| Subject |
Crayfish exoskeleton
DNA amplification DNA extraction |
|
| Title |
DNA extraction from crayfish exoskeleton
|
|
| Type |
Article
|
|