Protocol for <i style="">in vitro</i> somatic embryogenesis and regeneration of rice (<i style="">Oryza sativa</i> L.)
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Creator |
Verma, Dipti
Joshi, Rohit Shukla, Alok Kumar, Pramod |
|
| Date |
2011-11-30T09:36:34Z
2011-11-30T09:36:34Z 2011-12 |
|
| Identifier |
0975-1009 (Online); 0019-5189 (Print)
http://hdl.handle.net/123456789/13097 |
|
| Description |
958-963
Development of highly efficient and reproducible plant regeneration system has tremendous potential to provide improved technology to assist in genetic transformation of <i style="">indica</i> rice cultivars for their further exploitation in selection. For the development of a highly reproducible regeneration system through somatic embryogenesis, mature embryos of highly popular rice cultivars i.e., Govind (for rainfed areas), Pusa Basmati-1 (aromatic basmati) and Jaya (for irrigated areas) were used. Optimum callus formation (%) to MS medium supplemented with 2, 4-D was obtained at 12.0 m<i style="">M</i> in Govind, 14.0 m<i style="">M</i> in Jaya and 15.0 m<i style="">M</i> in Pusa Basmati-1. All the cultivars showed good proliferation on MS medium without hormone. In Govind, highest embryogenic response was observed in MS medium supplemented with 2, 4-D (0.4 m<i style="">M</i>) + kinetin (0.4 m<i style="">M</i>), while in Pusa Basmati-1 with 2, 4-D (0.4 m<i style="">M</i>) + kinetin (2.0 m<i style="">M</i>) and in Jaya on hormone-free MS medium. Excellent embryo regeneration in Govind was observed on MS medium supplemented with low concentrations (1.1 m<i style="">M</i>) of BAP or hormone-free MS medium, while in Pusa Basmati-1 and Jaya embryogenesis was observed on MS medium supplemented with higher concentration of BAP (2.2 m<i style="">M</i>). Similarly, maximum plantlets with proliferated roots were observed in Govind on hormone-free MS medium, while in Pusa Basmati-1 and Jaya on MS medium supplemented with high concentration of NAA (4.0 m<i style="">M</i>). Developed plantlets were further successfully acclimatized and grown under pot culture up to maturity. Further the yield potential of <i style="">in vitro</i> developed plants was accessed at par to the direct seeded one under pot culture. Present, protocol standardizes somatic embryogenesis and efficient regeneration of agronomically important, high yielding and diverse <i style="">indica</i> rice cultivars which can be utilized as an efficient tool for molecular studies and genetic transformation in future. |
|
| Language |
en_US
|
|
| Publisher |
NISCAIR-CSIR, India
|
|
| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
|
|
| Source |
IJEB Vol.49(12) [December 2011]
|
|
| Subject |
<i style="">Indica</i> rice
Mature embryo Somatic embryogenesis |
|
| Title |
Protocol for <i style="">in vitro</i> somatic embryogenesis and regeneration of rice (<i style="">Oryza sativa</i> L.)
|
|
| Type |
Article
|
|