Production and optimization of detergent compatible thermostable alkaline protease from <i>Bacillus cereus </i>FJ10
IR@NISCAIR: CSIR-NISCAIR, New Delhi - ONLINE PERIODICALS REPOSITORY (NOPR)
View Archive Info| Field | Value | |
| Creator |
Jabeen, Faiza
Qazi, Javed Iqbal |
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| Date |
2011-11-30T11:55:27Z
2011-11-30T11:55:27Z 2011-12 |
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| Identifier |
0975-1084 (Online); 0022-4456 (Print)
http://hdl.handle.net/123456789/13115 |
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| Description |
1042-1048
This study presents production and optimization of detergent compatible thermostable alkaline protease from <i>Bacillus </i><i>cereus </i>FJ10. Identification of isolate FJ10 that showed maximum growth when allowed to grow for 24 h at 50°C and pH 9.0 with 1% inoculum was confirmed by 16S rRNA gene sequencing. Proteases yielded maximum activity at 50°C having pH 10.0 and 20% substrate concentration. <i>B. cereus </i>FJ10 was partially purified by ammonium sulfate precipitation and dialysis. Both crude and partially purified enzymes destained blood spots on cotton fabrics. Alkaline and thermostable nature of bacterial proteases qualifies them for detergent formulation. |
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| Language |
en_US
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| Publisher |
NISCAIR-CSIR, India
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| Rights |
<img src='http://nopr.niscair.res.in/image/cc-license-sml.png'> <a href='http://creativecommons.org/licenses/by-nc-nd/2.5/in' target='_blank'>CC Attribution-Noncommercial-No Derivative Works 2.5 India</a>
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| Source |
JSIR Vol.70(12) [December 2011]
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| Subject |
<i>Bacillus cereus</i>
Detergent compatible enzyme Protease |
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| Title |
Production and optimization of detergent compatible thermostable alkaline protease from <i>Bacillus cereus </i>FJ10
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| Type |
Article
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