Thyroid hormone-induced protein (TIP) gene expression by 3,5,30-triiodothyronine in the ovarian follicle of perch (Anabas testudineus, Bloch):modulation of 3b-hydroxysteroid dehydrogenase/D5–D4-isomerase enzyme by TIP
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Thyroid hormone-induced protein (TIP) gene expression by
3,5,30-triiodothyronine in the ovarian follicle of perch
(Anabas testudineus, Bloch):modulation of 3b-hydroxysteroid
dehydrogenase/D5–D4-isomerase enzyme by TIP
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| Creator |
Datta, Malabika
Nagendra Prasad, R J Navneet, A K Roy, Sib Sankar Bhattacharya, Samir |
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| Subject |
Cell Biology & Physiology
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| Description |
Our previous reports had shown that 3,5,30-triiodothyronine (T3) induced the generation of a 52-kDa monomer protein, i.e., TIP
(thyroid hormone-induced protein) in the perch ovarian follicle. TIP, in turn, increased progesterone formation by stimulating
D5-3b-HSD activity (3b-hydroxysteroid dehydrogenase/D5–D4 isomerase) [Eur. J. Endocrinol. 134 (1996) 128–135; Gen. Comp.
Endocrinol. 113 (1999) 212–220]. In the present investigation, perch ovarian follicles were incubated in the absence (control) or the
presence of T3 or gonadotropin (GTH) or human chorionic gonadotropin (hCG). RNAs were isolated and allowed to hybridize
with a radiolabeled TIP oligonucleotide probe prepared on the basis of the N-terminal 17-amino-acid sequence of TIP. Only RNA
from T3-incubated follicles hybridized with the probe, while RNA from control or GTH- or hCG-incubated follicles did not hybridize
with the probe. The transcript size of TIP mRNA was �1.8 kb. mRNA isolated from T3-incubated ovarian follicles subjected
to in vitro translation and Western blot analysis clearly identified a 52-kDa protein which was not found with the mRNA from the
control follicles. However, both TIP and GTH stimulated progesterone secretion from perch ovarian follicles in vitro. GTH
stimulation of D5-3b-HSD was due to the stimulation of enzyme protein synthesis as a more than twofold increase in D5-3b-HSD
occurred in response to GTH. But TIP did not stimulate synthesis of D5-3b-HSD protein. However, in vitro incubation of D5-3b-
HSD enzyme with TIP in the presence of NAD and substrate (pregnenolone) greatly stimulated enzyme activity, while incubation
with GTH had no effect, indicating a modulation of D5-3b-HSD protein from a less active to a more active state by TIP. This has
been supported by another observation, in which TIP (52 kDa) and D5-3b-HSD (45 kDa) incubation resulted in a complex of
99 kDa. This suggests a protein–protein interaction in the process of D5-3b-HSD activation by TIP. The present work, therefore,
shows some new and interesting aspects of thyroid hormone regulation of the reproductive control mechanism. � 2002 Elsevier
Science (USA). All rights reserved.
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| Publisher |
Elsevier
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| Date |
2002
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/757/1/GENERAL_AND_COMPARATIVE_ENDOCRINOLOGY_126_(3)_334%2D341;2002[55].pdf
Datta, Malabika and Nagendra Prasad, R J and Navneet, A K and Roy, Sib Sankar and Bhattacharya, Samir (2002) Thyroid hormone-induced protein (TIP) gene expression by 3,5,30-triiodothyronine in the ovarian follicle of perch (Anabas testudineus, Bloch):modulation of 3b-hydroxysteroid dehydrogenase/D5–D4-isomerase enzyme by TIP. General and Comparative Endocrinology, 126 (3). pp. 334-341. |
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| Relation |
http://dx.doi.org/ 10.1016/S0016-6480(02)00009-6
http://www.eprints.iicb.res.in/757/ |
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