Involvement of Phosphatidylinositol 3 Kinase in the Progesterone-Induced Oocyte Maturation in Rana dybowskii
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Involvement of Phosphatidylinositol 3 Kinase
in the Progesterone-Induced Oocyte Maturation
in Rana dybowskii
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| Creator |
Ju, Jung-Won
Bandyopadhyay, Arun Im, Wook-Bin Chung, Jongkyeong Kwon, Hyuk Bang Choi, Hueng-Sik |
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| Subject |
Cell Biology & Physiology
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| Description |
Previously, we observed that 70-kDa ribosomal protein
S6 kinase (p70s6k) plays an essential role during the early
phase of oocyte maturation in Rana dybowskii. To investigate
further the early signal transduction components
involved in this process, the possible role of phosphatidylinositol-
3 kinase (PI3 kinase) during oocyte maturation
was examined. Progesterone-induced oocyte maturation
was significantly inhibited by wortmannin and
LY294002, specific inhibitors of PI3 kinase. In contrast,
protein kinase C activator 12-0-tetradecanoylphorbol-13-
acetate-induced oocyte maturation was not inhibited by
wortmannin. Protein synthesis was also significantly
suppressed by wortmannin treatment during oocyte maturation.
Moreover, PI3 kinase inhibitor suppressed progesterone-
induced phosphorylation of S6 kinase in a
dose-dependent manner. Likewise, PI3 kinase inhibitors
significantly inhibited the phosphorylation of mitogenactivated
protein (MAP) kinase which was increased during
oocyte maturation. Finally, progesterone-induced H1
kinase activity was also inhibited by PI3 kinase inhibitors
in a dose-dependent manner. Taken together, these results
suggest that PI3 kinase is an initial component of
the signal transduction pathway which precedes p70s6k,MAP kinase, and MPF production during progesteroneinduced
maturation of amphibian oocyte.
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| Publisher |
Elsevier
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| Date |
2002
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/758/1/GENERAL_AND_COMPARATIVE_ENDOCRINOLOGY_126(_2)_213%2D220;2002[60].pdf
Ju, Jung-Won and Bandyopadhyay, Arun and Im, Wook-Bin and Chung, Jongkyeong and Kwon, Hyuk Bang and Choi, Hueng-Sik (2002) Involvement of Phosphatidylinositol 3 Kinase in the Progesterone-Induced Oocyte Maturation in Rana dybowskii. General and Comparative Endocrinology, 126 (2). pp. 213-220. |
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| Relation |
http://dx.doi.org/10.1006/gcen.2002.7795
http://www.eprints.iicb.res.in/758/ |
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