Genetic and functional diversity among the antagonistic potential fluorescent Pseudomonada isolated from tea rhizosphere
IR@NEIST: CSIR-North East Institute of Science and Technology, Jorhat
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| Title |
Genetic and functional diversity among the antagonistic potential fluorescent Pseudomonada isolated from tea rhizosphere
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| Creator |
Saikia , R
Sarma, R K Yadav, A Bora, T C |
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| Subject |
Biotechnology
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| Description |
Twenty-five fluorescent pseudomonads from
rhizospheric soil of six tea gardens in four district of Upper
Assam, India were isolated and screened for antagonistic
activity against fungal pathogens such as Fusarium oxysporum
f. sp. raphani (For), Fusarium oxysporum f. sp. ciceri
(Foc), Fusarium semitectum (Fs), and Rhizoctonia solani
(Rs); and bacterial pathogens 14Staphylococcus aureus
(Sa), Escherichia coli (Ec), and Klebsiella pneumoniae
(Kp). Most of the isolates exhibited strong antagonistic
activity against the fungal pathogens and gram-positive
bacterium i.e. Staphylococcus aureus. Productions of siderophore,
salicylic acid (SA), hydrogen cyanide (HCN),
and cell wall-degrading enzyme (chitinase) were studied to
observe the possible mechanisms of antagonistic activity of
the isolates. Correlation between the antagonistic potentiality
of some isolates and their levels of production of
siderophore, salicylic acid, and hydrogen cyanide was
observed. Out of the 25 isolates, antibiotic-coding genes,
2,4-diacetylphloroglucinol (DAPG) and pyoluteorin (PLT)
were detected in the isolates, Pf12 and Pf373, respectively.
Genetic diversity of these fluorescent pseudomonads were
analyzed with reference to four strains of Pseudomonas
fluorescens NICM 2099T, P. aeruginosa MTCC 2582T,
P. aureofaciens NICM 2026T, and P. syringae MTCC 673T.
16S rDNA-RFLP analysis of these isolates using three tetra
cutter restriction enzymes (HaeIII, AluI and MspI) revealed
two distinct clusters. Cluster A comprised only two isolates
Pf141 and 24-PfM3, and cluster B comprised 23 isolates
along with four reference strains.
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| Publisher |
Springer
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| Date |
2011
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://neist.csircentral.net/11/1/2847.pdf
Saikia , R and Sarma, R K and Yadav, A and Bora, T C (2011) Genetic and functional diversity among the antagonistic potential fluorescent Pseudomonada isolated from tea rhizosphere. Current Microbiology, 62 (2). pp. 434-444. |
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| Relation |
http://neist.csircentral.net/11/
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