fMLP Receptor Stimulated Activation of Macrophage: Its Effect on Killing of Intracellular Leishmania donovani
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
fMLP Receptor Stimulated Activation of Macrophage:
Its Effect on Killing of Intracellular Leishmania donovani
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| Creator |
Dasgupta, Dalia
Chakraborty, Prasanta Basu, Mukul K |
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| Subject |
Drug Development/Diagnostics & Biotechnology
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| Description |
The fMLP receptor of peritoneal macrophages stimulated by fMLP grafted liposomes as
ligand, was analysed and compared with respective controls for its ability to promote killing
of intracellular Leishmania parasites. fMLP grafted liposomes show greater efficacy in killing
intracellular L. donoûani (MHOM�IN�1983�AG83) parasites in a time dependent manner
than free fMLP. fMLP grafted liposomes also release more active oxygen intermediates
and reactive nitrogen intermediates (O−2 , H2O2 , NO) than free fMLP. The key enzymes
PKC and PTK for the respiratory burst and nitric oxide generation were found to be
important in this fMLP receptor mediated signaling process as the enzyme specific inhibitors
viz. staurosporine, genistein and AG126 suppressed the leishmanicidal effect of fMLP
grafted liposomes. The above findings suggest that the fMLP receptor of macrophages
activates PKC and PTK mediated signalling that is responsible for the intracellular parasite
killing.
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| Publisher |
Kluwer
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| Date |
2000
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/808/1/BIOSCIENCE_REPORTS_20(_5)345%2D354;2000[15].pdf
Dasgupta, Dalia and Chakraborty, Prasanta and Basu, Mukul K (2000) fMLP Receptor Stimulated Activation of Macrophage: Its Effect on Killing of Intracellular Leishmania donovani. Bioscience Reports, 20 (5). p. 354. |
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| Relation |
http://dx.doi.org/10.1023/A:1010325916675
http://www.eprints.iicb.res.in/808/ |
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