Plasmid Curing from an Acidophilic Bacterium of the Genus Acidocella
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Plasmid Curing from an Acidophilic Bacterium of the Genus
Acidocella
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| Creator |
Ghosh, S
Mahapatra, Nitish R Ramamurthy, T Banerjee, Pataki C |
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| Subject |
Drug Development/Diagnostics & Biotechnology
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| Description |
Preservation of the acidophilic heterotroph, Acidocella sp. strain GS19h, at 4³C in stab culture eliminated all indigenous plasmids from
this bacterium. Growth at 42³C initially caused changes in the plasmid profile followed by total elimination of plasmids after 10 cycles of
growth. Concomitant to this loss of all plasmids, the cured derivatives became sensitive to CdSO4 and ZnSO4, and the MIC value of the
salts dropped from 1 M for each in the case of parental strain to 2 mM and 5 mM, respectively, suggesting plasmid-mediated inheritance of
metal resistance in this bacterium. The cured derivatives could not utilise lactose, indicating this metabolic activity to be plasmid-associated
in this strain. ß 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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| Publisher |
Elsevier
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| Date |
2000
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/818/1/FEMS_MICROBIOLOGY_LETTERS__183(_2)_271%2D274;2000[59].pdf
Ghosh, S and Mahapatra, Nitish R and Ramamurthy, T and Banerjee, Pataki C (2000) Plasmid Curing from an Acidophilic Bacterium of the Genus Acidocella. FEMS Microbiology Letters, 183 (2). pp. 271-274. |
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| Relation |
http://dx.doi.org/10.1016/S0378-1097(99)00640-0
http://www.eprints.iicb.res.in/818/ |
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