Modulation of CD11C+ Splenic Dendritic Cell Functions in Murine Visceral Leishmaniasis: Correlation with Parasite Replication in the Spleen
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Modulation of CD11C+ Splenic Dendritic Cell Functions in Murine Visceral
Leishmaniasis: Correlation with Parasite Replication in the Spleen
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| Creator |
Basu, A
Chakrabarty, G Saha, A Bandyopadhyay, Santu |
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| Subject |
Infectious Diseases and Immunology
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| Description |
BALB/c mice resolve Leishmania donovani infection in the liver over an 8±12-week period. However,
after an initial phase of 2±4 weeks where increases in parasite load are not readily detectable,
parasite numbers in the spleen begin to increase reaching maximum levels at 16 weeks postinfection.
Thereafter, parasite replication in the spleen is controlled and BALB/c mice maintain this
residual parasite load in the spleen for many months, without further increase. We evaluated
functions of CD11C+ splenic dendritic cells throughout the course of L. donovani infection in the
spleen of BALB/c mice. Unlike the dendritic cell (DC)-speci®c antigen DEC-205, CD11C was not
up-regulated on macrophages during visceral leishmaniasis. No appreciable impairment of splenic
DC functions was observed when this antigen-presenting cell subset was puri®ed from 30-day postinfected
mice. Signi®cant impairment in inducing allogeneic mixed lymphocyte reaction (MLR) and
presenting L. donovani antigens or keyhole limpet haemocyanin (KLH) to speci®c T cells was
observed with CD11C+ splenic DC puri®ed from 60-day post-infected mice. Functional impairment
of splenic DC at 60 days post-infection correlated with their reduced surface expression of major
histocompatibility complex (MHC) class II molecules, impairment of interleukin-12 (IL-12)
production and to their ability to suppress interferon-c (IFN-c) production by Leishmania antigenprimed
T cells. Of interest, the impairment of splenic DC in presenting Leishmania antigens or KLH
to speci®c T cells was corrected at 120 days post-infection, and correlated with their up-regulation of
MHC class II expression, IL-12 production, induction of IFN-c by Leishmania antigen-primed
T cells and the onset of control over splenic parasite replication in vivo. These results indicate that
functional integrity of DC may be important in controlling L. donovani infection.
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| Publisher |
Blackwell Publishing
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| Date |
2000
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/846/1/IMMUNOLOGY__99(2)_305%2D313;2000[61].pdf
Basu, A and Chakrabarty, G and Saha, A and Bandyopadhyay, Santu (2000) Modulation of CD11C+ Splenic Dendritic Cell Functions in Murine Visceral Leishmaniasis: Correlation with Parasite Replication in the Spleen. Immunology, 99 (2). pp. 305-313. |
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| Relation |
http://dx.doi.org/10.1046/j.1365-2567.2000.00939.x
http://www.eprints.iicb.res.in/846/ |
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