Macrophage Heterogeneity, Antigen Presentation, and Membrane Fluidity: Implications in Visceral Leishmaniasis
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Macrophage Heterogeneity, Antigen Presentation, and Membrane Fluidity: Implications in Visceral Leishmaniasis
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| Creator |
Sen, E
Chattopadhyay, S Bandyopadhyay, Santu De, Tripti Roy, Syamal |
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| Subject |
Chemistry
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| Description |
Morphological and functional heterogeneity of the splenic macrophage (Mf) population was studied in
Leishmania donovani (LD) infected BALB/c mice. On a discontinuous percoll gradient two distinct Mf
populations were separated. They differed significantly in size as evident from Scanning Electron
Microscopy (SEM). Morphologically, the bigger Mf (LM) showed surface projections, whereas the smaller
Mf (SM) was round. As regards the antigen-presenting abilities, the LM of infected animals showed
defective antigen-presenting abilities at a later stage of the disease, i.e. 6 months post infection (6I-LM) but
not earlier, whereas the SM population remained functionally intact throughout the course of the infection.
Further, the 6I-LM showed a much enhanced Ad status as compared to their controls. Interestingly, both the
6I-LM and the control set showed a comparable level of binding of a known Ad restricted peptide. Despite
the presence of sufficient Ad molecules and the ability to bind the appropriate peptide, 6I-LM were unable to
stimulate peptide specific T-cell hybridoma. Further, the 6I-LM showed an increase in membrane fluidity
and distorted morphology with membrane fissure and blebs as evident from SEM. It is possible that an
increase in the membrane fluidity may lead to the defective antigen-presenting ability of 6I-LM. Thus, the
LD infection functionally keep the 6I-LM out of antigen presentation and this may contribute to the defective
cell mediated immune response in leishmaniasis.
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| Date |
2001
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/864/1/SCANDINAVIAN_JOURNAL_OF_IMMUNOLOGY%2C_53_(2_)%2C_111%2D120%2C2001[60].pdf
Sen, E and Chattopadhyay, S and Bandyopadhyay, Santu and De, Tripti and Roy, Syamal (2001) Macrophage Heterogeneity, Antigen Presentation, and Membrane Fluidity: Implications in Visceral Leishmaniasis. Scandinavian Journal Of Immunology, 53 (2). pp. 111-120. |
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| Relation |
http://dx.doi.org/10.1046/j.1365-3083.2001.00856.x
http://www.eprints.iicb.res.in/864/ |
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