Interaction of Ascorbate and a-tocopherol Enhances Antioxidant Reserve of Erythrocytes during Anemia in Visceral Leishmaniasis
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Interaction of Ascorbate and a-tocopherol Enhances Antioxidant Reserve of Erythrocytes during Anemia
in Visceral Leishmaniasis
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| Creator |
Sen, Gargi
Mukhopadhaya, Rupa Ghosal, Jharna Biswas, Tuli |
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| Subject |
Cell Biology & Physiology
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| Description |
Visceral leishmaniasis (V.L.) is associated with enhanced lipid peroxidation along with impaired
function of antioxidant defense system in erythrocytes. The effect of chronic treatment with ascorbate
and
a-tocopherol was studied on erythrocytes in hamsters infected with Leishmania donovani. Combination
treatment with both antioxidants proved to be a potential suppressor of lipid hydroperoxide formation
as well as hypotonic osmotic lysis during the leishmanial infection. Positive correlations between
the depleted levels of erythrocyte ascorbate, GSH and
a-tocopherol exhibit proportionate
alterations in the nonenzymatic antioxidant levels at different stages of infection. Indirect measurement
of transmembrane electron transfer as ferricyanide reduction suggests an active participation
of endogenous contents of ascorbate and
a-tocopherol in the protection against oxidative damage of
membrane lipids. Cooperative behavior of both antioxidants in the ferricyanide reducing capacity was
further evinced by resealing the ghosts in presence of exogenous ascorbate and
a-tocopherol. Furthermore,
intravesicular ascorbate serves in the defense of extravesicular ferricyanide induced oxidation of
endogenous
a-tocopherol. The results suggest an interacting role of ascorbate and
a-tocopherol in
maintaining the antioxidant reserve of erythrocytes during anemia in V.L. © 2000 Elsevier Science
Inc. All rights reserved.
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| Publisher |
Elsevier
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| Date |
2000
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/867/1/LIFE_SCIENCES_67_(26)3181%2D3190;2000[5].pdf
Sen, Gargi and Mukhopadhaya, Rupa and Ghosal, Jharna and Biswas, Tuli (2000) Interaction of Ascorbate and a-tocopherol Enhances Antioxidant Reserve of Erythrocytes during Anemia in Visceral Leishmaniasis. Life Sciences, 67 (26). pp. 3181-3190. |
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| Relation |
http://dx.doi.org/10.1016/S0024-3205(00)00898-5
http://www.eprints.iicb.res.in/867/ |
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