Effect of growth environment on the stability of a recombinant shuttle plasmid, pCPPS-31, in Escherichia coli.
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
View Archive Info| Field | Value | |
| Title |
Effect of growth environment on the stability of a recombinant shuttle plasmid, pCPPS-31, in Escherichia coli.
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| Creator |
Gupta, R
Sharma, P Vyas, V V |
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| Subject |
QR Microbiology
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| Description |
The effect of environmental growth conditions on the segregational stability of a recombinant Escherichia coli-Bacillus subtilis shuttle plasmid pCPPS-31 which expresses carboxymethyl cellulase (CMCase) and contains the neomycin resistance gene (Ner), was studied in E. coli DH5 alpha. The stability increased with the decrease in medium complexity. Lowering culture temperatures had a negative effect on stability. Stability was maximum at 100 rpm and a medium/flask volume ratio of 1:10. A pH range of 5-8 had no significant effect on stability. The recombination (rec) and gyrase (gyr) backgrounds of E. coli hosts had no apparent effect on plasmid stability. The plasmid was structurally stable under all circumstances.
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| Publisher |
Elsevier Science
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| Date |
1995-07-15
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://crdd.osdd.net/open/394/1/vyas95.pdf
Gupta, R and Sharma, P and Vyas, V V (1995) Effect of growth environment on the stability of a recombinant shuttle plasmid, pCPPS-31, in Escherichia coli. Journal of biotechnology, 41 (1). pp. 29-37. ISSN 0168-1656 |
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| Relation |
http://www.sciencedirect.com/science/article/pii/016816569500049V
http://crdd.osdd.net/open/394/ |
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