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A 150-kDa molecule of macrophage membrane stimulates interleukin-2 and interferon-gamma production and proliferation of ovalbumin-specific CD4+ T cells.

IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh


Field	Value

Title	A 150-kDa molecule of macrophage membrane stimulates interleukin-2 and interferon-gamma production and proliferation of ovalbumin-specific CD4+ T cells.

Creator	Agrewala, J N Vinay, D S Joshi, A Mishra, G C

Subject	QR180 Immunology

Description	In the present study, we describe the potential co-stimulatory role of a macrophage membrane-associated protein of 150 kDa (M150). The protein was isolated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and was found to be a single molecule on two-dimensional gel electrophoresis. The molecule was re-constituted in phosphatidyl choline vesicles and tested for its ability to promote the proliferation and the secretion of lymphokines from T helper (Th) cells. The reconstituted M150 induced a significant proliferation of anti-CD3 monoclonal antibody (mAb)-stimulated ovalbumin-specific CD4+ T cells. Further, Th cells activated with this molecule in the presence of anti-CD3 mAb mainly secreted interleukin (IL)-2 and interferon-gamma but not IL-4. M150 could not promote the proliferation of Th cells, or lymphokine secretion in the absence of anti-CD3 mAb. These observations suggest that M150 acts by selectively activating a Th1-like immune response.

Publisher	Wiley

Date	1994-09

Type	Article PeerReviewed

Format	application/pdf

Identifier	http://crdd.osdd.net/open/405/1/Agrewala1994%28S%29.pdf Agrewala, J N and Vinay, D S and Joshi, A and Mishra, G C (1994) A 150-kDa molecule of macrophage membrane stimulates interleukin-2 and interferon-gamma production and proliferation of ovalbumin-specific CD4+ T cells. European journal of immunology, 24 (9). pp. 2092-7. ISSN 0014-2980

Relation	http://onlinelibrary.wiley.com/doi/10.1002/eji.1830240924/pdf http://crdd.osdd.net/open/405/