Calculation of antibody and antigen concentrations from ELISA data using a graphical method.
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
View Archive Info| Field | Value | |
| Title |
Calculation of antibody and antigen concentrations from ELISA data using a graphical method.
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| Creator |
Raghava, G.P.S.
Joshi, A K Agrewala, J N |
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| Subject |
QR180 Immunology
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| Description |
A graphical method for determining the concentration of either the antibody or the antigen from ELISA data is presented in the form of a GWBASIC program. In the program, ELISAEQ, optical densities (OD) obtained from a 96-well ELISA plate can be input either directly by interfacing a microplate reader to the computer or manually. The program uses standard sample data, and selects the semilogarithmic linear range. Over this range, a least-squares method is used to determine the concentrations of interest. In addition, a hyperbolic interpolation formula is derived over the entire range for estimating the antibody or antigen concentration of the unknown samples whose OD is beyond the linear range.
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| Publisher |
Elsevier Science
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| Date |
1992-08-30
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| Type |
Article
PeerReviewed |
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| Format |
text/html
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| Identifier |
http://crdd.osdd.net/open/422/1/raghava92.mht
Raghava, G.P.S. and Joshi, A K and Agrewala, J N (1992) Calculation of antibody and antigen concentrations from ELISA data using a graphical method. Journal of immunological methods, 153 (1-2). pp. 263-4. ISSN 0022-1759 |
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| Relation |
http://www.sciencedirect.com/science/article/pii/002217599290330V
http://crdd.osdd.net/open/422/ |
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