Novel assay for screening rifamycin B-producing mutants.
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
View Archive Info| Field | Value | |
| Title |
Novel assay for screening rifamycin B-producing mutants.
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| Creator |
Vohra, R M
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| Subject |
QR Microbiology
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| Description |
A simple method that allows easy identification of rifamycin B-producing strains is described. This method involves the use of an enzyme, rifamycin oxidase, which converts inactive rifamycin B to active rifamycin S. In this method, colonies to be tested are grown in pairs. The two colonies are then transferred to two plates seeded with a sensitive strain of Staphylococcus aureus, one plate of which contains the enzyme rifamycin oxidase. All paired colonies which show a larger inhibition zone diameter on the enzyme-containing plate are identified as rifamycin B producers.
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| Publisher |
ASM
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| Date |
1992-01
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://crdd.osdd.net/open/426/1/vohra92.pdf
Vohra, R M (1992) Novel assay for screening rifamycin B-producing mutants. Applied and environmental microbiology, 58 (1). pp. 403-4. ISSN 0099-2240 |
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| Relation |
http://aem.asm.org/content/58/1/403.full.pdf
http://crdd.osdd.net/open/426/ |
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