Serine protease inhibitor mediated peptide bond re-synthesis in diverse protein molecules.
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
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| Title |
Serine protease inhibitor mediated peptide bond re-synthesis in diverse protein molecules.
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| Creator |
Sharma, Amit
Radha Kishan, K V |
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| Subject |
QR Microbiology
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| Description |
Protease inhibitors have been extensively used in research to prevent unwanted degradation of proteins during purification and analysis. Here, we report a remarkable discovery of protease inhibitor mediated reformation of peptide bonds by the serine protease inhibitor, PMSF in a diverse set of proteolyzed molecules. Interestingly, the religation reaction in the presence of PMSF occurs in a very short time period and with very high yields of the religated product. We also investigate the plausible mechanism of such a reaction and demonstrate through biochemical studies and X-ray crystallography that proximity of reacting termini is essential for the feasibility of this reaction.
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| Publisher |
Elsevier Science
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| Date |
2011-11-04
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://crdd.osdd.net/open/445/1/radha11.pdf
Sharma, Amit and Radha Kishan, K V (2011) Serine protease inhibitor mediated peptide bond re-synthesis in diverse protein molecules. FEBS letters, 585 (21). pp. 3465-70. ISSN 1873-3468 |
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| Relation |
http://www.sciencedirect.com/science/article/pii/S0014579311007423
http://crdd.osdd.net/open/445/ |
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