A catalase-peroxidase for oxidation of β-lactams to their (R)-sulfoxides.
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
View Archive Info| Field | Value | |
| Title |
A catalase-peroxidase for oxidation of β-lactams to their (R)-sulfoxides.
|
|
| Creator |
Sanger, Shefali
Pal, Mohan Moon, Lomary S Jolly, R S |
|
| Subject |
QR Microbiology
|
|
| Description |
In this communication we report for the first time a biocatalytic method for stereoselective oxidation of β-lactams, represented by penicillin-G, penicillin-V and cephalosporin-G to their (R)-sulfoxides. The method involves use of a bacterium, identified as Bacillus pumilis as biocatalyst. The enzyme responsible for oxidase activity has been purified and characterized as catalase-peroxidase (KatG). KatG of B. pumilis is a heme containing protein showing characteristic heme spectra with soret peak at 406nm and visible peaks at 503 and 635nm. The major properties that distinguish B. pumilis KatG from other bacterial KatGs are (i) it is a monomer and contains one heme per monomer, whereas KatGs of other bacteria are dimers or tetramers and have low heme content of about one per dimer or two per tetramer and (ii) its 12-residue, N-terminal sequence obtained by Edman degradation did not show significant similarity with any of known KatGs.
|
|
| Publisher |
Elsevier Science
|
|
| Date |
2011-09-21
|
|
| Type |
Article
PeerReviewed |
|
| Relation |
http://www.sciencedirect.com/science/article/pii/S0960852411013319
http://crdd.osdd.net/open/446/ |
|
| Identifier |
Sanger, Shefali and Pal, Mohan and Moon, Lomary S and Jolly, R S (2011) A catalase-peroxidase for oxidation of β-lactams to their (R)-sulfoxides. Bioresource technology. ISSN 1873-2976 (In Press)
|
|