Reductive dehalogenation mediated initiation of aerobic degradation of 2-chloro-4-nitrophenol (2C4NP) by Burkholderia sp. strain SJ98.
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
View Archive Info| Field | Value | |
| Title |
Reductive dehalogenation mediated initiation of aerobic degradation of 2-chloro-4-nitrophenol (2C4NP) by Burkholderia sp. strain SJ98.
|
|
| Creator |
Pandey, Janmejay
Heipieper, Hermann J Chauhan, Archana Arora, Pankaj Kumar Prakash, Dhan Takeo, Masahiro Jain, R K |
|
| Subject |
QR Microbiology
|
|
| Description |
Burkholderia sp. strain SJ98 (DSM 23195) was previously isolated and characterized for degradation and co-metabolic transformation of a number nitroaromatic compounds. In the present study, we evaluated its metabolic activity on chlorinated nitroaromatic compounds (CNACs). Results obtained during this study revealed that strain SJ98 can degrade 2-chloro-4-nitrophenol (2C4NP) and utilize it as sole source of carbon, nitrogen, and energy under aerobic conditions. The cells of strain SJ98 removed 2C4NP from the growth medium with sequential release of nearly stoichiometric amounts of chloride and nitrite in culture supernatant. Under aerobic degradation conditions, 2C4NP was transformed into the first intermediate that was identified as p-nitrophenol by high-performance liquid chromatography, LCMS-TOF, and GC-MS analyses. This transformation clearly establishes that the degradation of 2C4NP by strain SJ98 is initiated by "reductive dehalogenation"; an initiation mechanism that has not been previously reported for microbial degradation of CNAC under aerobic conditions.
|
|
| Publisher |
Springer Science
|
|
| Date |
2011-11
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://crdd.osdd.net/open/466/1/jain11.pdf
Pandey, Janmejay and Heipieper, Hermann J and Chauhan, Archana and Arora, Pankaj Kumar and Prakash, Dhan and Takeo, Masahiro and Jain, R K (2011) Reductive dehalogenation mediated initiation of aerobic degradation of 2-chloro-4-nitrophenol (2C4NP) by Burkholderia sp. strain SJ98. Applied microbiology and biotechnology, 92 (3). pp. 597-607. ISSN 1432-0614 |
|
| Relation |
http://www.springerlink.com/content/p6378j3847216134/fulltext.pdf
http://crdd.osdd.net/open/466/ |
|