A novel disposable electrochemical immunosensor for phenyl urea herbicide diuron.
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
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| Title |
A novel disposable electrochemical immunosensor for phenyl urea herbicide diuron.
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| Creator |
Sharma, Priyanka
Sablok, Kavita Bhalla, Vijayender Suri, C Raman |
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| Subject |
QD Chemistry
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| Description |
A disposable electrochemical immunosensor has been developed for the determination of phenyl urea herbicide-diuron using a low cost laser ablated gold electrodes (LC-LAGE) fabricated on polystyrene substrate. The electrodes were electrochemically deposited with prussian blue-gold nanoparticle (PB-GNP) film, and a competitive inhibition immunoassay was performed on LC-LAGE by using a specific hapten-protein conjugate. The binding of available diuron specific antibody on conjugate coated electrode was detected using alkaline phosphatase rabbit anti-IgG antibody. The addition of 1-naphthyl phosphate substrate resulted in the production of electrochemically active product, 1-naphthol, which was monitored using square wave voltammetry technique. The assay exhibited an excellent sensitivity and specificity showing the dynamic response range between 1 ppt and 10 ppm for diuron with detection limit around 1 ppt. This study provides insight into development of a rapid and high-throughput screening of pesticides in environmental samples at a very low cost.
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| Publisher |
Elsevier Science
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| Date |
2011-06-15
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://crdd.osdd.net/open/470/1/suri11.1.pdf
Sharma, Priyanka and Sablok, Kavita and Bhalla, Vijayender and Suri, C Raman (2011) A novel disposable electrochemical immunosensor for phenyl urea herbicide diuron. Biosensors & bioelectronics, 26 (10). pp. 4209-12. ISSN 1873-4235 |
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| Relation |
http://www.sciencedirect.com/science/article/pii/S0956566311001758
http://crdd.osdd.net/open/470/ |
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