Gold nanoparticles catalyzed chemiluminescence immunoassay for detection of herbicide 2,4-dichlorophenoxyacetic acid.
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
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| Title |
Gold nanoparticles catalyzed chemiluminescence immunoassay for detection of herbicide 2,4-dichlorophenoxyacetic acid.
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| Creator |
Boro, Robin C
Kaushal, Jyotsna Nangia, Yogesh Wangoo, Nishima Bhasin, Aman Suri, C Raman |
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| Subject |
QD Chemistry
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| Description |
We present a novel immunoassay format utilizing the catalytic properties of gold nanoparticles in the luminol-silver nitrate-gold nanoparticle based chemiluminescence (CL) system for the detection of widely used herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). Highly sensitive anti-2,4-D antibody was produced and conjugated with gold nanoparticles of various sizes. In the present assay format, employing a competitive inhibition approach, a well-characterized hapten-protein conjugate (2,4-D-BSA) was used to coat the microtiter plates. The analyte (2,4-D) was pre-incubated with anti-2,4-D antibody labeled with gold nanoparticles and added to each well of the microtiter plate. The gold label triggered the reaction between luminol and silver nitrate generating a luminescence signal at 425 nm. Under the optimized conditions, the CL based immunoassay showed the detection limit of 2,4-D in standard water samples around 3 ng mL(-1). The CL based immunoassay format, based on gold nanoparticles as a catalyst, could be used as a fast screening methodology (<30 min) for pesticide detection.
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| Publisher |
RSC Publishing
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| Date |
2011-05-21
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://crdd.osdd.net/open/474/1/suri11.pdf
Boro, Robin C and Kaushal, Jyotsna and Nangia, Yogesh and Wangoo, Nishima and Bhasin, Aman and Suri, C Raman (2011) Gold nanoparticles catalyzed chemiluminescence immunoassay for detection of herbicide 2,4-dichlorophenoxyacetic acid. The Analyst, 136 (10). pp. 2125-30. ISSN 1364-5528 |
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| Relation |
http://pubs.rsc.org/en/content/articlepdf/2011/an/c0an00810a
http://crdd.osdd.net/open/474/ |
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