Role of Swi6/HP1 self-association-mediated recruitment of Clr4/Suv39 in establishment and maintenance of heterochromatin in fission yeast.
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
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| Title |
Role of Swi6/HP1 self-association-mediated recruitment of Clr4/Suv39 in establishment and maintenance of heterochromatin in fission yeast.
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| Creator |
Haldar, Swati
Saini, Ashok Nanda, Jagpreet S Saini, Sharanjot Singh, Jagmohan |
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| Subject |
QD Chemistry
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| Description |
Swi6/HP1, an evolutionarily conserved protein, is critical for heterochromatin assembly in fission yeast and higher eukaryotes. In fission yeast, histone deacetylation by histone deacetylases is thought to be followed by H3-Lys-9 methylation by the histone methyltransferase Clr4/Suv39H1. H3-Lys-9-Me2 interacts with the chromodomain of Swi6/HP1. Swi6/HP1 is thought to act downstream of Clr4/Suv39, and further self-association of Swi6/HP1 is assumed to stabilize the heterochromatin structure. Here, we show that the self-association-defective mutant of Swi6 does not interact with Clr4. It not only fails to localize to heterochromatin loci but also interferes with heterochromatic localization of H3-Lys-9-Me2 (and thereby Clr4) and the endogenous Swi6 in a dominant negative manner. Thus, self-association of Swi6/HP1 helps in binding to and recruitment of Clr4 and thereby in establishment and maintenance of heterochromatin by a concerted rather than a sequential mechanism.
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| Publisher |
ASBMB
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| Date |
2011-03-18
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://crdd.osdd.net/open/484/1/jagmohan11.pdf
Haldar, Swati and Saini, Ashok and Nanda, Jagpreet S and Saini, Sharanjot and Singh, Jagmohan (2011) Role of Swi6/HP1 self-association-mediated recruitment of Clr4/Suv39 in establishment and maintenance of heterochromatin in fission yeast. The Journal of biological chemistry, 286 (11). pp. 9308-20. ISSN 1083-351X |
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| Relation |
http://www.jbc.org/content/early/2011/01/11/jbc.M110.143198.full.pdf+html
http://crdd.osdd.net/open/484/ |
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