Pentoxifylline induces apoptosis in vitro in cutaneous T cell lymphoma (HuT-78) and enhances FasL mediated killing by upregulating Fas expression.
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
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| Title |
Pentoxifylline induces apoptosis in vitro in cutaneous T cell lymphoma (HuT-78) and enhances FasL mediated killing by upregulating Fas expression.
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| Creator |
Rishi, Loveena
Gahlot, Satindra Kathania, Mahesh Majumdar, Sekhar |
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| Subject |
QD Chemistry
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| Description |
Constitutive nuclear factor-kappaB (NF-kappaB) is known to play an important role in the survival of HuT-78 cells, a cutaneous T cell lymphoma (CTCL) cell line. Here, we have demonstrated that pentoxifylline (PTX), a phosphodiesterase inhibitor, can trigger a series of events leading to apoptosis in HuT-78 cells without affecting NF-kappaB. Apoptosis was ascertained by sub-G1 peak analysis and TUNEL assay. Apoptosis induced by PTX in HuT-78 cells involved mitochondrial hyperpolarization, cytochrome c release, caspase-3 activation and PARP cleavage. Further, it was found that PTX treatment downregulated Bcl-xl and c-FLIP expression without affecting constitutive NF-kappaB but upregulated activator protein-1 (AP-1). Low concentration of PTX upregulated Fas and TRAIL expression in HuT-78 cells. In addition, PTX can act as a scavenger of reactive oxygen intermediate and it could enhance FasL mediated killing in HuT-78 cells. Our results taken together indicated that PTX may be a potential agent for killing CTCL cells.
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| Publisher |
Elsevier Science
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| Date |
2009-01-01
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://crdd.osdd.net/open/579/1/majumdar09.pdf
Rishi, Loveena and Gahlot, Satindra and Kathania, Mahesh and Majumdar, Sekhar (2009) Pentoxifylline induces apoptosis in vitro in cutaneous T cell lymphoma (HuT-78) and enhances FasL mediated killing by upregulating Fas expression. Biochemical pharmacology, 77 (1). pp. 30-45. ISSN 1873-2968 |
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| Relation |
http://www.sciencedirect.com/science/article/pii/S000629520800645X
http://crdd.osdd.net/open/579/ |
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