Nitric oxide induces apoptosis in cutaneous T cell lymphoma (HuT-78) by downregulating constitutive NF-kappaB.
IR@IMTECH: CSIR-Institute of Microbial Technology, Chandigarh
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| Title |
Nitric oxide induces apoptosis in cutaneous T cell lymphoma (HuT-78) by downregulating constitutive NF-kappaB.
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| Creator |
Rishi, Loveena
Dhiman, Rohan Raje, Manoj Majumdar, Sekhar |
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| Subject |
QD Chemistry
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| Description |
Constitutive active NF-kappaB have been shown to protect cutaneous T cell lymphoma (CTCL) cells from apoptosis. In the present study, we have studied the cytotoxic potential of nitric oxide generating compound, sodium nitroprusside (SNP) on CTCL cell line, HuT-78. Treatment of cells with SNP resulted in decrease in mitochondrial membrane potential, cytochrome c release, activation of caspase-3 and poly (ADP ribose) polymerase cleavage. SNP treatment inhibited activation of NF-kappaB in a concentration dependent manner. SNP increased the expression of IkappaBalpha without affecting the phosphorylation of IkappaBalpha. Downregulation of NF-kappaB by SNP decreased p65 nuclear translocation as evident by confocal fluorescence microscopy. Further it was found that SNP treatment caused downregulation of Bcl-2 family member (Bcl-xl) in HuT-78 cells. Thus, we have provided evidence that SNP induces apoptosis in CTCL cell line, HuT-78 by downregulating constitutive NF-kappaB and thereby Bcl-xl expression.
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| Publisher |
Elsevier Science
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| Date |
2007-08
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://crdd.osdd.net/open/633/1/majumdar07.pdf
Rishi, Loveena and Dhiman, Rohan and Raje, Manoj and Majumdar, Sekhar (2007) Nitric oxide induces apoptosis in cutaneous T cell lymphoma (HuT-78) by downregulating constitutive NF-kappaB. Biochimica et biophysica acta, 1770 (8). pp. 1230-1239. ISSN 0006-3002 |
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| Relation |
http://www.sciencedirect.com/science/article/pii/S0304416507001109
http://crdd.osdd.net/open/633/ |
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