Primaquine-induced differential gene expression analysis in mice liver using DNA microarrays
IR@CDRI: CSIR-Central Drug Research Institute, Lucknow
View Archive Info| Field | Value | |
| Creator |
Noel, Sanjeev
Sharma, Sharad Shanker, Rishi Rath, Srikanta Kumar |
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| Date |
2007-12-27T05:32:41Z
2007-12-27T05:32:41Z 2007 |
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| Identifier |
Toxicology 239 (2007) 96-107
http://hdl.handle.net/123456789/59 |
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| Description |
Primaquine (PQ). a clinically important derivative of 8-aminoquinoline used against the hepatic stages (hypnozoites) of Plasmodium vivax
and Plasmodium ova Ie. was studied to evaluate and compare between mRNA expression. and biochemical and histological parameters of
hepatic stress in adult Swiss mice (Mus musculus). Following single oral dose of PQ (40 mglkg. bw). alanine aminotransferase (ALT) and
aspartate aminotransferase (AST) along with hematoxylin and eosin stained liver sections did not show any signs of hepatic stress at 6. 12 and 24
h except for ALT activity at 6 h. However. analysis at RNA transcript level revealed consistent and significant deregulation (p<0.01 and twofold)
of 16 probes corresponding to important cellular processes such as protein transportation. transcription regulation. intracellular signaling.
protein synthesis, hematopoiesis, cell adhesion and cell proliferation. Pathway analysis identified large number of affected genes corresponding
to 40 Gene Ontology terms having a z score greaibr than 2. These results indicate that PQ at high doses may affect gene expression in liver and may produce undesirable outcomes if consumed for longer durations.
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| Format |
1210289 bytes
application/pdf |
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| Language |
en
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| Relation |
CDRI Communication no. 7220.
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| Subject |
8-Aminoquinoline
Differential gene expression Microarray Primaquine Toxicogenomics |
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| Title |
Primaquine-induced differential gene expression analysis in mice liver using DNA microarrays
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| Type |
Article
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