Effect of Altered Polar Headgroup of Phosphatidylethanolamines on Transbilayer Aminophospholipid Distribution in Sonicated Vesicles
IR@CDRI: CSIR-Central Drug Research Institute, Lucknow
View Archive Info| Field | Value | |
| Creator |
Kumar, Ajay
Gupta, C M |
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| Date |
2008-03-23T07:08:53Z
2008-03-23T07:08:53Z 1983 |
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| Identifier |
Biochimica el Biophysica Acta (1983), 730, 1-9
http://hdl.handle.net/123456789/114 |
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| Description |
The transbilayer aminophospholipid distributions in small unilamellar vesicles comprising of phosphatidylethanolamine
or its analogs (bearing modifications in the polar headgroup) and egg hosphatidylcholine were ascertained using trinitrobenzenesulfonic acid as external membrane probe. These vesicles, containing
10-30 mol% phosphatidylethanolamine or its analogs, were formed by sonication and fractionated by centrifugation. Phosphatidylethanolamine at low concentrations (10 mol%) preferentially localized in the outer monolayer. This preference appeared to be reversed at higher phosphatidylethanolamine concentrations (30 mol%). Unlike this finding, phosphatidylethanolamine bearing ethyl, phenyl and benzyl substituents at the
carbon atom adjacent to the amino group distributed mainly in the outer surface irrespective of their concentrations. Similar results were obtained when the phosphate and amino groups were separated by three methylene residues. These observations suggest that the effective polar headgroup volume and/or
hydrogen-bonding capacity of phospholipids are the important factors that determine their distribution in small unilamellar vesicles.
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3518994 bytes
application/pdf |
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| Language |
en
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| Relation |
CDRI Communication No. 2718
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| Subject |
Phospholipid distribution
Phosphatidylethanolamine analog Aminophospholipid Headgroup Membrane vesicle |
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| Title |
Effect of Altered Polar Headgroup of Phosphatidylethanolamines on Transbilayer Aminophospholipid Distribution in Sonicated Vesicles
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| Type |
Article
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