CSIR Central

Two-hybrid-based analysis of protein-protein interactions of the yeast multidrug resistance protein, Pdr5p

IR@CDRI: CSIR-Central Drug Research Institute, Lucknow

View Archive Info
 
 
Field Value
 
Creator Rao, G S
Bachhawat, A K
Gupta, C M
 
Date 2008-10-23T20:54:15Z
2008-10-23T20:54:15Z
2002
 
Identifier Funct.Integer.Genomics 1,357-366 (2002)
http://hdl.handle.net/123456789/207
 
Description The ATP binding cassette (ABC) transporters are a large family of proteins responsible for the translocation of a variety of compounds across the membranes of both prokaryotes and eukaryotes. The inter-protein and intra-protein interactions in these traffic ATPase are still only poorly understood. In the present study we describe, for the first time, an extensive yeast two-hybrid (Y2H)-based analysis of the interactions of the cytoplasmic loops of the yeast pleiotropic drug resistance (Pdr) protein. Pdr5p, an ABC transporter of Saccharomyces cerevisiae. Four of the major cytosolic loops that have been predicted for this protein [including the two nucleotide-binding domain (NBD)-containing loops and the cytosolic C-terminal region] were subjected to an extensive inter-domain interaction study in addition to being used as baits to identify potential interacting proteins within the cell using the Y2H system. Results of these studies have revealed that the first cytosolic loop (CL1) – containing the first NBD domain – and also the C-terminal region of Pdr5p interact with several candidate proteins. The possibility of an interaction between the CL1 loops of two neighboring Pdr5p molecules was also indicated, which could possibly have implications for dimerization of this protein.
 
Format 5658292 bytes
application/pdf
 
Language en
 
Relation CDRI Communication no. 6166
 
Subject Yeast two-hybrid system
ABC transporter Pdr5p NBD1
 
Title Two-hybrid-based analysis of protein-protein interactions of the yeast multidrug resistance protein, Pdr5p
 
Type Article