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A monoclonal IgM directed against immunodominant catalase B of cell wall of Aspergillus fumigatus exerts anti-A. fumigatus activities

IR@CDRI: CSIR-Central Drug Research Institute, Lucknow


Field	Value

Creator	Chaturvedi, A K Kumar, Rohitashw Kumar, Awanit Shukla, P K

Date	2010-11-03T05:04:10Z 2010-11-03T05:04:10Z 2009

Identifier	Mycoses, 2009, 52 (6) 524-533 http://hdl.handle.net/123456789/631

Description	Aspergillus fumigatus a ubiquitous fungus has been reported to cause human diseases like allergic pulmonary aspergillosis, aspergilloma, and invasive infection. Invasive aspergillosis is one of the most important causes of mortality in patients with hematological malignancies and in bone marrow transplant recipients. The currently available antifungal drugs mostly target the ergosterol synthesis. However, there are a few antifungals, such as echinocandin and glycolipid papulacandin, with which inhibition of cell wall glucans biosynthesis leads to cessation of growth and cell lysis. Limited spectrum and emergence of resistance has become a serious problem with available antifungals. Therefore, an alternative approach is required for successful treatment of mycoses. In the present study, immunogenic protein profile of A. fumigatus cell wall was generated using 2D-gel electrophoresis and three hybridomas producing monoclonal antibodies (IgM) were selected after fusion experiments. Of these three monoclonal antibodies, MAb-7 exhibited potent in vitro inhibitory activity, which was confirmed by MTT assay, FACS analysis, and immuno-fluorescence studies, and the protein was identified as catalase B using MALDI-TOF-MS.

Format	493768 bytes application/pdf

Language	en

Relation	CDRI Communication no. 7092

Subject	ASPERGILLUS FUMIGATUS MONOCLONAL ANTIBODIES CATALASE B FLOWCYTOMETRY

Title	A monoclonal IgM directed against immunodominant catalase B of cell wall of Aspergillus fumigatus exerts anti-A. fumigatus activities

Type	Article