Antiproliferative effects of Curcumin plus Centchroman in MCF-7 and MDA MB-231 cells
IR@CDRI: CSIR-Central Drug Research Institute, Lucknow
View Archive Info| Field | Value | |
| Creator |
Zaidi, Deeba
Singh, Neetu Ahmad, I Z Sharma, Ramesh Balapure, A K |
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| Date |
2011-08-18T11:15:58Z
2011-08-18T11:15:58Z 2011 |
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| Identifier |
International Journal of Pharmacy and Pharmaceutical Sciences (2011) 3(2), 212-216
http://hdl.handle.net/123456789/709 |
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| Description |
Cancer co-chemotherapy involves the advantages of lowered individual drug doses with minimal chances of development of resistance. We investigated whether low (1-5µM) and high (10-50µM) doses of Curcumin (CUR) synergize with putative anti-breast cancer agent Centchroman (CC) in vitro. ER+ve MCF-7 and ER–ve MDA MB-231 Human Breast Cancer Cells (HBCCs) were employed for cytotoxicity assays and ROS generation studies. Normal Human Gingival Fibroblasts (hGF) demonstrate the safety of administered drugs. Drugs alone (1-25µM CC; 1-50µM CUR) show dose-dependent cytotoxicity on HBCCs but are non-toxic to hGF. 1-20µM CC plus 1-5µM CUR display absence of synergy. 20µM CUR plus CC at IC50 doses of ~10/20µM in MCF-7/MDA MB-231 cells respectively displays additive effect. Additionally, CUR at IC50 dose (~35µM) plus CC at 5µM in MCF-7 cells and 10µM in MDA MB-231 respectively display the reduction of IC50 of CC as a ROS mediated combinatorial effect.
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| Format |
467930 bytes
application/pdf |
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| Language |
en
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| Relation |
cddricommunicationno.8013
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| Subject |
ER+ve MCF-7
ER–ve MDA MB-231 Human Breast Cancer Cells (HBCCs) Centchroman (CC) |
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| Title |
Antiproliferative effects of Curcumin plus Centchroman in MCF-7 and MDA MB-231 cells
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| Type |
Article
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