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Sugar Receptor Mediated Drug delivery to Macrophages in the Therapy of Experimental Visceral Leishmaniasis

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata


Field	Value

Title	Sugar Receptor Mediated Drug delivery to Macrophages in the Therapy of Experimental Visceral Leishmaniasis

Creator	Chakraborty, Prasanta Bhaduri, Amar Nath Das, Pijush K

Subject	Infectious Diseases and Immunology

Description	Methotrexate (MTX) conjugate of a neoglycoprotein, mannosyl bovine serum albumin, containing an average of 30 moles of MTX per mole of neoglycoprotein was taken up efficiently by murine peritoneal macrop hages through cell surface mannosy 1 receptors. The conjugate strongly inhibits the growth of Leishmania donovani inside macrophages. with 50% inhibitory dose of 0.11 pglml MTX, which makes it 100 times more active than free MTX (50% inhibitory dose of 12.1 yg/ml). MTX conjugated to BSA or other non-specific neoglycoproteins like galactose-BSA and glucose- BSA have leishmanicidal effects comparable to free MTX. Moreover, in a murine model of experimental visceral leishmaniasis, the drug conjugate reduced the spleen parasite burden by more than 85% in a 30 day model whereas the same concentration of free drug caused little effect. The results demonstrate that neoglycoproteins may be useful as carriers for receptor mediated drug delivery to treat macrophage associated diseases.

Publisher	Elsevier

Date	1990

Type	Article PeerReviewed

Format	application/pdf

Identifier	http://www.eprints.iicb.res.in/951/1/BIOCHEMICAL_AND_BIOPHYSICAL_RESEARCH_COMMUNICATIONS__166(_1_)_404%2D410;199%2D[54].pdf Chakraborty, Prasanta and Bhaduri, Amar Nath and Das, Pijush K (1990) Sugar Receptor Mediated Drug delivery to Macrophages in the Therapy of Experimental Visceral Leishmaniasis. Biochemical and Biophysical Research Communications, 166 (1). pp. 404-410.

Relation	http://dx.doi.org/10.1016/0006-291X(90)91959-V http://www.eprints.iicb.res.in/951/