Toxic Interaction of Thionine to Deoxyribonucleic Acids: Elucidation of the Sequence Specificity of Binding with Polynucleotides
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Toxic Interaction of Thionine to Deoxyribonucleic Acids: Elucidation of the Sequence Specificity of Binding with Polynucleotides
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| Creator |
Paul, Puja
Kumar, G Suresh |
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| Subject |
Chemistry
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| Description |
The sequence specificity of the intercalative DNA damage of the phenothiazine dye thionine has been investigated by absorbance, fluorescence, circular dichroism and viscosity studies using four synthetic polynucleotides, poly(dA-dT)·poly(dA-dT), poly(dA)·poly(dT), poly(dG-dC)·poly(dG-dC) and poly(dG)·poly(dC). Strong hypochromic-bathochromic effects in absorbance and quenching in fluorescence
were observed that showed strong binding of thionine to these polynucleotides. Scatchard plots revealed non-cooperative binding and analysis by McGhee–von Hippel equation provided the affinity values in the order of 105M−1. The binding clearly revealed the high preference of thionine to the alternating GC sequences followed by the homo GC sequences. The AT polynucleotides had lower binding affinities but the alternating AT sequences had higher affinity compared to the homo stretches. The results of ferrocyanide quenching studies in fluorescence and viscosity experiments conclusively proved the intercalation of thionine while circular dichroic studies provided evidence for the structural perturbations
associated with the sequence specific intercalative binding. The sequence specificity of the intercalative
damage of thionine to deoxyribonucleic acid is advanced from this study.
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| Date |
2010
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/957/1/3_jhm_2010.pdf
Paul, Puja and Kumar, G Suresh (2010) Toxic Interaction of Thionine to Deoxyribonucleic Acids: Elucidation of the Sequence Specificity of Binding with Polynucleotides. Journal of Hazardous Materials, 184 (1-3). pp. 620-626. |
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| Relation |
http://dx.doi.org/10.1016/j.jhazmat.2010.08.081
http://www.eprints.iicb.res.in/957/ |
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