Chenopodium Album seed Extract-induced Sperm Cell Death: Exploration of a Plausible Pathway
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Chenopodium Album seed Extract-induced Sperm Cell Death:
Exploration of a Plausible Pathway
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| Creator |
Kumar, Shrabanti
Chatterjee, Ratna Dolai, Subhankar Adak, Subrata Kabir, S N Banerjee, Sukdeb Mondal, Nirup B |
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| Subject |
Chemistry
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| Description |
Background: This study was conducted for to explore the plausible pathway of Chenopodium album seed extract (CAE)-mediated sperm
cell death.
Study Design: The role of CAE for its spermicidal action was assessed by (a) measuring lipid peroxidation, protein carbonyl content and
intracellular glutathione content in CAE exposed sperm cells; (b) assaying antioxidant enzymes like catalase and superoxide dismutase
(SOD); (c) analyzing protein expressions by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis; (d)
fluorimetric measurement of intracellular H2O2 level and generation of reactive oxygen species (ROS) in CAE-treated sperm cells; and (e)
DNA ladder formation study.
Results: CAE-induced sperm death is due to (a) lipid peroxidation of the sperm cell membrane, oxidation of some critical cellular proteins
and depletion of intracellular reduced gluthathione, indicating production of ROS; (b) activation of Mn-SOD and inactivation of catalase
favoring endogenous accumulation of H2O2; (c) generation of O2 U− at an enhanced rate during oxidative stress as evidenced by increased Mn-
SOD activity and protein expression; (d) accumulation of ROS in spermatozoa reflected in the fluorimetric experiments; and (e) increased
production of O2 U− and H2O2 induced apoptosis-like death in sperm cells as observed by DNA ladder formation.
Conclusion: The sperm death mediated by CAE is due to oxidative damage of cellular macromolecules by in situ generation of ROS.
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/962/1/CONTRACEPTION___77(_6)_456%2D462;2008[72].pdf
Kumar, Shrabanti and Chatterjee, Ratna and Dolai, Subhankar and Adak, Subrata and Kabir, S N and Banerjee, Sukdeb and Mondal, Nirup B (2008) Chenopodium Album seed Extract-induced Sperm Cell Death: Exploration of a Plausible Pathway. Contraception, 77 (6). pp. 456-462. |
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| Relation |
http://dx.doi.org/10.1016/j.contraception.2008.02.006
http://www.eprints.iicb.res.in/962/ |
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