Activation of Proinflammatory Response in Human Intestinal Epithelial Cells Following Vibrio Cholerae Infection Through PI3K/Akt Pathway
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Activation of Proinflammatory Response in Human
Intestinal Epithelial Cells Following Vibrio Cholerae
Infection Through PI3K/Akt Pathway
|
|
| Creator |
Bandyopadhyay, Arunava
Bhowmick, Swati Chaudhuri, Keya |
|
| Subject |
Molecular & Human Genetics
|
|
| Description |
Vibrio cholerae activates proinflammatory response in cultured intestinal epithelial cells. In this study, we demonstrate
that V. cholerae O395 infection of intestinal epithelial cells results in the activation of Akt. Inhibition of Akt significantly
decreases IL-1a, IL-6, and TNF-a production in V. cholerae infected Int407 cells. Analysis of the mechanisms
of Akt influences on cytokine response demonstrates that Akt promotes NF-kB activation. We have extended these findings
to show that Akt activation may be regulated by bacterial genes associated with virulence, adherence, or motility. Insertion
mutants in the virulence genes coding for CtxA, ToxT, and OmpU of V. cholerae modulate the activation of PI3K/
Akt signaling pathway, whereas an aflagellate non-motile mutant (O395FLAN) and a adherent and less motile mutant
(O395Y3N/O395Y4N) of V. cholerae both show very significant down-regulation of Akt activity in Int407 cells. Together,
these observations indicate that Akt promotes proinflammatory cytokine production by V. cholerae infected human intestinal epithelial cells through its influences on NF-kB.
|
|
| Date |
2009
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/966/1/6_CJM_2009.pdf
Bandyopadhyay, Arunava and Bhowmick, Swati and Chaudhuri, Keya (2009) Activation of Proinflammatory Response in Human Intestinal Epithelial Cells Following Vibrio Cholerae Infection Through PI3K/Akt Pathway. Canadian Journal of Microbiology (CJM), 55 (11). pp. 1310-1318. |
|
| Relation |
http://dx.doi.org/10.1139/W09-093
http://www.eprints.iicb.res.in/966/ |
|