Involvement of PI3 Kinase and MAP Kinase in IGF-I- and Insulin-induced Oocyte Maturation in Cyprinus Carpio
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Involvement of PI3 Kinase and MAP Kinase in IGF-I- and Insulin-induced Oocyte Maturation in Cyprinus Carpio
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| Creator |
Paul, Sudipta
Pramanick, Kousik Kundu, Sourav Bandyopadhyay, Arun Mukherjee, Dilip |
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| Subject |
Drug Development/Diagnostics & Biotechnology
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| Description |
Previously, we observed that in vitro germinal vesicle breakdown (GVBD) in Cyprinus carpio oocytes was
induced by recombinant human insulin-like growth factor-I (IGF-I) and bovine insulin (b-insulin) and
this induction was steroid-independent. To investigate further the early signal transduction components
involved in this process, the possible role of phosphatidylinositol 3-kinase (PI3 kinase) during oocyte
maturation was examined. IGF-I- and b-insulin-induced oocyte maturation was significantly inhibited
by Wortmannin and LY294002, two mechanistically different specific inhibitors of PI3 kinase. IGF-I and
b-insulinwere shown to activate PI3 kinase after 90 min of their treatment. Both IGF-I and b-insulinwere
found to activate cdc2 kinase at 21 h of treatment. We examined the relative involvement of PI3 kinase,
MAP kinase and cdc2 kinase in IGF-I- and b-insulin-induced oocyte maturation in C. carpio. MAP kinase
was rapidly phosphorylated and activated (30–150 min) in response to exposure of the oocytes with
IGF-I and b-insulin. This response preceded the phosphorylation and activation of cdc2 by several hours
(almost 19 h). A potent and selective inhibitor of MEK, PD98059, the protein kinase that phosphorylates
and activate MAP kinase, blocked the phosphorylation and activation of MAP kinase and cdc2 kinase
and GVBD induction. Likewise, PI3 kinase inhibitors strongly inhibited phosphorylation and activation of
MAP kinase, which was increased during oocyte maturation. Taken together, these results suggest that
PI3 kinase is an initial component of the signal transduction pathway which precedes MAP kinase, and
MPF activation during IGF-I- and b-insulin-induced oocyte maturation in C. carpio.
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| Date |
2009
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/973/1/8_MCE_2009.pdf
Paul, Sudipta and Pramanick, Kousik and Kundu, Sourav and Bandyopadhyay, Arun and Mukherjee, Dilip (2009) Involvement of PI3 Kinase and MAP Kinase in IGF-I- and Insulin-induced Oocyte Maturation in Cyprinus Carpio. Molecular and Cellular Endocrinology, 309 (1-2). pp. 93-100. |
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| Relation |
http://dx.doi.org/10.1016/j.mce.2009.05.014
http://www.eprints.iicb.res.in/973/ |
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