Thermodynamic Profiles of The DNA Binding of Benzophenanthridines Sanguinarine and Ethidium: A Comparative Study with Sequence Specific Polynucleotides
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Thermodynamic Profiles of The DNA Binding of Benzophenanthridines Sanguinarine and Ethidium: A Comparative Study with Sequence Specific Polynucleotides
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| Creator |
Hossain, Maidul
Kumar, G Suresh |
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| Subject |
Chemistry
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| Description |
Energetics of the binding of two known classical DNA intercalating molecules, ethidium and sanguinarine
with four sequence specific polynucleotides, poly(dG-dC).poly(dG-dC), poly(dG).poly(dC), poly(dAdT).
poly(dA-dT), and poly(dA).poly(dT) have been compared under identical conditions. The binding of
both the molecules was characterized by strong stabilization of the polynucleotides against thermal
strand separation in optical melting as well as differential scanning calorimetry studies. Isothermal titration
calorimetry results revealed that the binding of both sanguinarine and ethidium to poly(dG-dC).poly(
dG-dC), poly(dA-dT).poly(dA-dT), and poly(dG).poly(dC) was exothermic and favoured by negative
enthalpy changes. On the other hand, the binding of both molecules to poly(dA).poly(dT) was endothermic
and entropy driven. The binding affinity values obtained from isothermal titration calorimetry data
was in close proximity to that derived from thermal melting data. The heat capacity changes obtained
from temperature dependence of the enthalpy change gave negative values in the range (�0.4 to
1.25) kJ � mol�1 � K�1 for the binding of ethidium and sanguinarine to these polynucleotides. The variations
in the values indicate important differences in the formation of the complexes. New insights into
the energetics and specificity aspects of interaction of these molecules to DNA have emerged from these
studies.
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| Date |
2010
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/975/1/19_JCT_2010.pdf
Hossain, Maidul and Kumar, G Suresh (2010) Thermodynamic Profiles of The DNA Binding of Benzophenanthridines Sanguinarine and Ethidium: A Comparative Study with Sequence Specific Polynucleotides. J. Chem. Thermodynamics, 42 (10). pp. 1273-1280. |
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| Relation |
http://dx.doi.org/10.1016/j.jct.2010.05.005
http://www.eprints.iicb.res.in/975/ |
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