IL-1b Expression in Int407 is Induced by Flagellin of Vibrio cholerae through TLR5 Mediated Pathway
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
IL-1b Expression in Int407 is Induced by Flagellin
of Vibrio cholerae through TLR5 Mediated Pathway
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| Creator |
Bandyopadhaya, Arunava
Sarkar, Madhubanti Chaudhuri, Keya |
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| Subject |
Molecular & Human Genetics
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| Description |
Vibrio cholerae, a noninvasive enteric bacterium, causing inflammatory diarrheal disease cholera, is associated with the secretion of
proinflamammatory cytokines including IL-1b in cultured epithelial cells. Incubation of Int407 with live V. cholerae resulted in increased
IL-1b mRNA expression as early as 2 h of infection, reached a peak at �3.5 h and decreased thereafter. The identity of the effector
molecule(s) is largely unknown. The bacterial culture supernatant showed IL-1b stimulating activity. An engineered aflagellate
V. cholerae flaA mutant (O395FLAN) resulted in highly reduced level of IL-1b expression in Int407. The crude flagellar protein of
V. cholerae as well as recombinant FlaA induced IL-1b expression in Int407. Infection of Toll-like receptor 5 (TLR5) transfected HeLa
cells with O395FLAN showed reduced expression of IL-1b compared to wild-type. Unlike wild-type V. cholerae, O395FLAN did not
activate the NF-kB while the recombinant flagellin could activate NF-kB. Finally, the mitogen activated protein kinases (ERK1 and 2,
p38) were phosphorylated in wild-type and recombinant flagellin treated Int407 cells and inhibition of the p38 and ERK pathways
significantly decreased the IL-1b response induced by wild-type V. cholerae as well as recombinant flagellin. Our data clearly indicate that
flagellin of V. cholerae could induce IL-1b expression by recognizing TLR5 that activate NF-kB and MAP kinase in Int407.
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/982/1/MICROBIAL_PATHOGENESIS__44_(_6_)__524%2D536;2008[75].pdf
Bandyopadhaya, Arunava and Sarkar, Madhubanti and Chaudhuri, Keya (2008) IL-1b Expression in Int407 is Induced by Flagellin of Vibrio cholerae through TLR5 Mediated Pathway. Microbial Pathogenesis, 44 (6). pp. 524-536. |
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| Relation |
http://dx.doi.org/10.1016/j.micpath.2008.01.004
http://www.eprints.iicb.res.in/982/ |
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