Optimization of Covalent Antibody Immobilization on Macroporous Silicon Solid Supports
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Optimization of Covalent Antibody Immobilization on Macroporous Silicon Solid Supports
|
|
| Creator |
Das, R. Dev
Maji, S Das, Sumantra RoyChaudhuri, C |
|
| Subject |
Cell Biology & Physiology
|
|
| Description |
In this paper, optimization of the protocol for covalent antibody immobilization on macroporous silicon
solid supports of various porosities, which is recently being employed as a promising substrate for
biosensors, has been reported. Covalent binding of antibody has been carried out by silanization and
crosslinker attachment on the substrate. For maximum antibody immobilization on macroporous silicon,
all the individual processes have been separately optimized for the first time in terms of treatment
time, pH, concentration, incubation time and others with the help of optical density measurements. The
optimum treatment of the surface after every step has been further reconfirmed by detailed EDX analysis,
SEM measurements and contact angle measurements. It has been observed that the density of antibody
binding increases with increasing porosity and for a 70% porosity macroporous sample it is almost three
times more than that of planar silicon which is significantly higher than the previous comparative reports
on planar silicon and macroporous silicon. The amount of properly oriented HIgG antibodies has been
estimated by quantification of the alkaline phosphate conjugated protein A binding by optical density
measurements.
|
|
| Date |
2010
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/985/1/27_asc_2010.pdf
Das, R. Dev and Maji, S and Das, Sumantra and RoyChaudhuri, C (2010) Optimization of Covalent Antibody Immobilization on Macroporous Silicon Solid Supports. Applied Surface Science, 256 (20). pp. 5867-5875. |
|
| Relation |
http://dx.doi.org/10.1016/j.apsusc.2010.03.066
http://www.eprints.iicb.res.in/985/ |
|