Analysis of Free and Bound NADPH in Aqueous Extract of Human placenta Used as Wound Healer.
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Analysis of Free and Bound NADPH in Aqueous Extract of Human
placenta Used as Wound Healer.
|
|
| Creator |
De, Debashree
Chakraborty, Piyali Datta Bhattacharyya, Debasish |
|
| Subject |
Structural Biology & Bioinformatics
|
|
| Description |
NADPH is an important biomolecule involved in cellular regeneration. The distribution of free and bound
NADPH in aqueous extract of human placenta used as a potent wound healer has been analyzed. Quantification
from fluorescence and immuno-affinity chromatography indicates that 75.1±2.2% of NADPH
present in the extract exists as free nucleotide or bound to very small peptides or amino acids whereas
the rest remains bound to large peptides. Inability to dissociate the bound form of the nucleotide from
the large peptides using urea or guanidium hydrochloride indicates that the binding is covalent. Identification
of a fragmented mass of m/z 382.94 (nicotinamide + sugar + phosphate) from the NADPH-peptide
conjugates supported the intactness of the nicotinamide moiety. Glutathione reductase assay indicated
that 95.2±3.5% of the total NADPH pool of the extract can act as cosubstrate of the enzyme. This indicates
that while a major fraction of free NADPH of the extract is easily available for cellular processes, the rest
can also function locally where the conjugated peptides are deposited.
|
|
| Date |
2009
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/989/1/20_JC_2009.pdf
De, Debashree and Chakraborty, Piyali Datta and Bhattacharyya, Debasish (2009) Analysis of Free and Bound NADPH in Aqueous Extract of Human placenta Used as Wound Healer. Journal of Chromatography B, 877 (24). pp. 2435-2442. |
|
| Relation |
http://dx.doi.org/:10.1016/j.jchromb.2009.05.018
http://www.eprints.iicb.res.in/989/ |
|