Gallic Acid Prevents Nonsteroidal Anti-Inflammatory Drug-Induced Gastropathy in Rat by Blocking Oxidative Stress and Apoptosis
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Gallic Acid Prevents Nonsteroidal Anti-Inflammatory Drug-Induced Gastropathy in Rat by Blocking Oxidative Stress and Apoptosis
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| Creator |
Pal, Chinmay
Bindu, Samik Dey, Sumanta Alam, Athar Goyal, Manish Iqbal, Mohd. Shameel Maity, Pallab Adhikari, Susanta Bandyopadhyay, Uday |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Nonsteroidal anti-inflammatory drug (NSAID)-induced oxidative stress plays a critical role in gastricmucosal cell
apoptosis and gastropathy. NSAIDs induce the generation of hydroxyl radical (•OH) through the release of free
iron, which plays an important role in developing gastropathy. Thus, molecules having both iron-chelating and
antiapoptotic properties will be beneficial in preventing NSAID-induced gastropathy. Gallic acid (GA), a
polyphenolic natural product, has the capacity to chelate free iron.Here,we report that GA significantly prevents,
as well as heals, NSAID-induced gastropathy. In vivo, GA blocks NSAID-mediated mitochondrial oxidative stress
by preventing mitochondrial protein carbonyl formation, lipid peroxidation, and thiol depletion. In vitro, GA
scavenges free radicals and blocks •OH-mediated oxidative damage. GA also attenuates gastric mucosal cell
apoptosis in vivo aswell as in vitro in cultured gastricmucosal cells as evident fromthe TUNEL assay.GA prevents
NSAID-induced activation of caspase-9, a marker for the mitochondrial pathway of apoptosis, and restores
NSAID-mediated collapse of the mitochondrial transmembrane potential and dehydrogenase activity. Thus, the
inhibition of mitochondrial oxidative stress by GA is associated with the inhibition of NSAID-induced
mitochondrial dysfunction and activation of apoptosis in gastric mucosal cells, which are responsible for gastric
injury or gastropathy.
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| Date |
2010
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/997/1/30_frbm_2010.pdf
Pal, Chinmay and Bindu, Samik and Dey, Sumanta and Alam, Athar and Goyal, Manish and Iqbal, Mohd. Shameel and Maity, Pallab and Adhikari, Susanta and Bandyopadhyay, Uday (2010) Gallic Acid Prevents Nonsteroidal Anti-Inflammatory Drug-Induced Gastropathy in Rat by Blocking Oxidative Stress and Apoptosis. Free Radical Biology & Medicine, 49 (2). pp. 258-267. |
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| Relation |
http://dx.doi.org/10.1016/j.freeradbiomed.2010.04.013
http://www.eprints.iicb.res.in/997/ |
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