Macroporous Silicon Based Simple and Efficient Trapping Platform for Electrical Detection of Salmonella Typhimurium Pathogens
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Macroporous Silicon Based Simple and Efficient Trapping Platform for Electrical Detection of Salmonella Typhimurium Pathogens
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| Creator |
Deb Das, R
Roychoudhury, C Maji, S Das, Sumantra Saha, H |
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| Subject |
Cell Biology & Physiology
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| Description |
A thermally oxidized macroporous silicon substrate with simple electrode structure without interdigitated
electrode configuration has been reported for the detection of Salmonella typhimurium pathogens by
electrical impedance measurement using antibody–antigen binding method. Macroporous silicon which
has been fabricated by anodizing silicon in HF and DMF solution is a regular network of pores of 1–2�m
diameters. This has been thermally oxidized to yield the surface hydrophilic for antibody immobilization
as well as provide suitable electrical insulation of the metal contacts from the underlying conducting
silicon substrate. The macroporous silicon surface has been immobilized by Salmonella specific antibody
and has been tested with different concentration of S. typhimurium pathogens in phosphate buffer solution
(PBS). It has been found that such macroporous silicon substrates is capable of detecting down to
103 CFU/ml in pure culture using a 3mm×1mm electrode structure with a wide spacing of 1mm. The
selectivity of the macroporous silicon substrates with reference to S. typhimurium has been tested to be
satisfactory by carrying out controlled experiments with Escherichia coli O157:H7.
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| Date |
2009
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/1001/1/28_BB_2009.pdf
Deb Das, R and Roychoudhury, C and Maji, S and Das, Sumantra and Saha, H (2009) Macroporous Silicon Based Simple and Efficient Trapping Platform for Electrical Detection of Salmonella Typhimurium Pathogens. Biosensors and Bioelectronics, 24 (11). pp. 3215-3222. |
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| Relation |
http://dx.doi.org/:10.1016/j.bios.2009.04.014
http://www.eprints.iicb.res.in/1001/ |
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